Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer
Joint Authors
Muthukumarappan, Kasiviswanathan
Gibbons, William R.
Eckard, Anahita Dehkhoda
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-14
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Although lignocellulosic materials have a good potential to substitute current feedstocks used for ethanol production, conversion of these materials to fermentable sugars is still not economical through enzymatic hydrolysis.
High cost of cellulase has prompted research to explore techniques that can prevent from enzyme deactivation.
Colloidal proteins of casein can form monolayers on hydrophobic surfaces that alleviate the de-activation of protein of interest.
Scanning electron microscope (SEM), fourier transform infrared spectroscopy (FT-IR), capillary electrophoresis (CE), and Kjeldahl and BSA protein assays were used to investigate the unknown mechanism of action of induced cellulase activity during hydrolysis of casein-treated biomass.
Adsorption of casein to biomass was observed with all of the analytical techniques used and varied depending on the pretreatment techniques of biomass.
FT-IR analysis of amides I and II suggested that the substructure of protein from casein or skim milk were deformed at the time of contact with biomass.
With no additive, the majority of one of the cellulase mono-component, 97.1 ± 1.1, was adsorbed to CS within 24 h, this adsorption was irreversible and increased by 2% after 72 h.
However, biomass treatment with skim-milk and casein reduced the adsorption to 32.9% ± 6.0 and 82.8% ± 6.0, respectively.
American Psychological Association (APA)
Eckard, Anahita Dehkhoda& Muthukumarappan, Kasiviswanathan& Gibbons, William R.. 2012. Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer. BioMed Research International،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-991876
Modern Language Association (MLA)
Eckard, Anahita Dehkhoda…[et al.]. Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer. BioMed Research International No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-991876
American Medical Association (AMA)
Eckard, Anahita Dehkhoda& Muthukumarappan, Kasiviswanathan& Gibbons, William R.. Analysis of Casein Biopolymers Adsorption to Lignocellulosic Biomass as a Potential Cellulase Stabilizer. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-991876
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991876