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Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp
Joint Authors
Zhang, Yuyu
Yuan, Jinduo
Zhu, Wenxing
Yang, Guiwen
An, Liguo
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-10
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Flavobacterium columnare is a bacterial pathogen causing high mortality rates for many freshwater fish species.
Fish vaccination with a safe and effective vaccine is a potential approach for prevention and control of fish disease.
Here, in order to produce bacterial ghost vaccine, a specific Flavobacterium lysis plasmid pBV-E-cat was constructed by cloning PhiX174 lysis gene E and the cat gene with the promoter of F.
columnare into the prokaryotic expression vector pBV220.
The plasmid was successfully electroporated into the strain F.
columnare G4cpN22 after curing of its endogenous plasmid.
F.
columnare G4cpN22 ghosts (FCGs) were generated for the first time by gene E-mediated lysis, and the vaccine potential of FCG was investigated in grass carp (Ctenopharyngodon idellus) by intraperitoneal route.
Fish immunized with FCG showed significantly higher serum agglutination titers and bactericidal activity than fish immunized with FKC or PBS.
Most importantly, after challenge with the parent strain G4, the relative percent survival (RPS) of fish in FCG group (70.9%) was significantly higher than FKC group (41.9%).
These results showed that FCG could confer immune protection against F.
columnare infection.
As a nonliving whole cell envelope preparation, FCG may provide an ideal alternative to pathogen-based vaccines against columnaris in aquaculture.
American Psychological Association (APA)
Zhu, Wenxing& Yang, Guiwen& Zhang, Yuyu& Yuan, Jinduo& An, Liguo. 2012. Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991888
Modern Language Association (MLA)
Zhu, Wenxing…[et al.]. Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991888
American Medical Association (AMA)
Zhu, Wenxing& Yang, Guiwen& Zhang, Yuyu& Yuan, Jinduo& An, Liguo. Generation of Biotechnology-Derived Flavobacterium columnare Ghosts by PhiX174 Gene E-Mediated Inactivation and the Potential as Vaccine Candidates against Infection in Grass Carp. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991888
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991888