Interaction between Pheromone and Its Receptor of the Fission Yeast Schizosaccharomyces pombe Examined by a Force Spectroscopy Study
Joint Authors
Abe, Ryohei
Kiyosaki, Shoichi
Sasuga, Shintaro
Osada, Toshiya
Ikai, Atsushi
Nikaido, Osamu
Sekiguchi, Hiroshi
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-21
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Interaction between P-factor, a peptide pheromone composed of 23 amino acid residues, and its pheromone receptor, Mam2, on the cell surface of the fission yeast Schizosaccharomyces pombe was examined by an atomic force microscope (AFM).
An AFM tip was modified with P-factor derivatives to perform force curve measurements.
The specific interaction force between P-factor and Mam2 was calculated to be around 120 pN at a probe speed of 1.74 μm/s.
When the AFM tip was modified with truncated P-factor derivative lacking C-terminal Leu, the specific interaction between the tip and the cell surface was not observed.
These results were also confirmed with an assay system using a green fluorescent protein (GFP) reporter gene to monitor the activation level of signal transduction following the interaction of Mam2 with P-factor.
American Psychological Association (APA)
Sasuga, Shintaro& Abe, Ryohei& Nikaido, Osamu& Kiyosaki, Shoichi& Sekiguchi, Hiroshi& Ikai, Atsushi…[et al.]. 2012. Interaction between Pheromone and Its Receptor of the Fission Yeast Schizosaccharomyces pombe Examined by a Force Spectroscopy Study. BioMed Research International،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991906
Modern Language Association (MLA)
Sasuga, Shintaro…[et al.]. Interaction between Pheromone and Its Receptor of the Fission Yeast Schizosaccharomyces pombe Examined by a Force Spectroscopy Study. BioMed Research International No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-991906
American Medical Association (AMA)
Sasuga, Shintaro& Abe, Ryohei& Nikaido, Osamu& Kiyosaki, Shoichi& Sekiguchi, Hiroshi& Ikai, Atsushi…[et al.]. Interaction between Pheromone and Its Receptor of the Fission Yeast Schizosaccharomyces pombe Examined by a Force Spectroscopy Study. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-991906
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991906