Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression
Joint Authors
Logroscino, Giandomenico
Neri, Giovanni
Saulnier, Nathalie
Gambotto, Andrea
Barba, Marta
Lattanzi, Wanda
Michetti, Fabrizio
Pirozzi, Filomena
Vitali, Tiziana
Natale, Maria Teresa
Robbins, Paul D.
Pola, Enrico
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Multipotent mesenchymal stem cells with extensive self-renewal properties can be easily isolated and rapidly expanded in culture from small volumes of amniotic fluid.
These cells, namely, amniotic fluid-stromal cells (AFSCs), can be regarded as an attractive source for tissue engineering purposes, being phenotypically and genetically stable, plus overcoming all the safety and ethical issues related to the use of embryonic/fetal cells.
LMP3 is a novel osteoinductive molecule acting upstream to the main osteogenic pathways.
This study is aimed at delineating the basic molecular events underlying LMP3-induced osteogenesis, using AFSCs as a cellular model to focus on the molecular features underlying the multipotency/differentiation switch.
For this purpose, AFSCs were isolated and characterized in vitro and transfected with a defective adenoviral vector expressing the human LMP3.
LMP3 induced the successful osteogenic differentiation of AFSC by inducing the expression of osteogenic markers and osteospecific transcription factors.
Moreover, LMP3 induced an early repression of the kruppel-like factor-4, implicated in MSC stemness maintenance.
KLF4 repression was released upon LMP3 silencing, indicating that this event could be reasonably considered among the basic molecular events that govern the proliferation/differentiation switch during LMP3-induced osteogenic differentiation of AFSC.
American Psychological Association (APA)
Barba, Marta& Pirozzi, Filomena& Saulnier, Nathalie& Vitali, Tiziana& Natale, Maria Teresa& Logroscino, Giandomenico…[et al.]. 2012. Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression. BioMed Research International،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-991912
Modern Language Association (MLA)
Barba, Marta…[et al.]. Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression. BioMed Research International No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-991912
American Medical Association (AMA)
Barba, Marta& Pirozzi, Filomena& Saulnier, Nathalie& Vitali, Tiziana& Natale, Maria Teresa& Logroscino, Giandomenico…[et al.]. Lim Mineralization Protein 3 Induces the Osteogenic Differentiation of Human Amniotic Fluid Stromal Cells through Kruppel-Like Factor-4 Downregulation and Further Bone-Specific Gene Expression. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-991912
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991912