Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip
Joint Authors
Zheng, Weiping
Wang, Yungen
Bian, Mei-lu
Tao, Pingping
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Persistent infection of high-risk human papillomavirus (HPV) has been recognized as the direct cause of cervical carcinoma.
Therefore, detection and genotyping of HPV are important to cervical-cancer screening.
In this study, we have evaluated the efficacy of flow-through hybridization and gene chip (HybriMax) on HPV genotyping through comparison of the results with Hybrid Capture II (HC-II) and in situ hybridization (ISH).
591 women were classified into 6 groups according to their histological diagnoses.
The overall accordance rate on 13 types of HPV genotypes between HybriMax and HC-II were 92.5% and 100% in the cancer group.
The overall accordance was excellent with the Kappa index (KI) of 0.814.
The value of KI in each group was 0.750 (normal cytological diagnosis), 0.781 (chronic cervicitis), 0.80 (condyloma acuminatum), 0.755 (cervical intraepithelial neoplasia (CIN) I), 0.723 (CIN II), and 0.547 (CIN III) (0.75>KI>0.4, good; KI≥0.75, excellent).
The 10 most common HPV subtype detected by HybriMax were 16, 52/58, 18, 33, 31, 81, 53, 68, and 66 in patients, and 16, 68, 18, 52, 58, 11, 53, 31/39, and 33 in normal controls.
In conclusion, HybriMax is an efficient method for HPV genotyping and more suitable for clinical use.
American Psychological Association (APA)
Tao, Pingping& Zheng, Weiping& Wang, Yungen& Bian, Mei-lu. 2012. Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip. BioMed Research International،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991960
Modern Language Association (MLA)
Tao, Pingping…[et al.]. Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip. BioMed Research International No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-991960
American Medical Association (AMA)
Tao, Pingping& Zheng, Weiping& Wang, Yungen& Bian, Mei-lu. Sensitive HPV Genotyping Based on the Flow-Through Hybridization and Gene Chip. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-991960
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991960