Inhibitory Effects of the Attenuated Salmonella typhimurium Containing the IL-2 Gene on Hepatic Tumors in Mice
Joint Authors
Yin, Qiang
Ha, Xiao-qin
Zhang, Yuan-yuan
Hui, Ling
Peng, Jun-hua
Zhao, Hong-bin
Wang, Mei-liang
Dong, Ju-zi
Deng, Zhi-yun
Zhao, Yong
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
To observe the inhibitory effects of an attenuated S.
typhimurium strain carrying IL-2 gene (TPI) on hepatoma cell line (HepG2) and transplanted tumors in mice.
TPI, TPG (an attenuated S.
typhimurium strain carrying green fluorescent protein gene), and TP (an attenuated S.
typhimurium strain) strains were transfected into HepG2 cells.
At 48h after transfecting, the transfection rate was 82.58 ± 1.74%.
The expression level of IL-2 was (99.5 ± 12.2) ng/1×106 cells.
Compared with TPG, TP, and normal mouse groups, the proportion of CD4+ T and CD8+ T cells in the blood from the TPI group was higher, the levels of IgM and IgG1 were significantly increased, and the proliferation activity of splenic lymphocyte was significantly stronger.
The transplanted tumor weight in the TPI group was significantly smaller than that in the other two groups.
The infiltration of lymphocytes increased in the tumor from TPI group mice.
TPI was effectively transfected into cancer cells, which expressed the protein of interest.
Oral administration of TPI prolonged survival of mice transplanted with hepatoma cell tumours.
American Psychological Association (APA)
Ha, Xiao-qin& Yin, Qiang& Zhao, Hong-bin& Hui, Ling& Wang, Mei-liang& Peng, Jun-hua…[et al.]. 2012. Inhibitory Effects of the Attenuated Salmonella typhimurium Containing the IL-2 Gene on Hepatic Tumors in Mice. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991963
Modern Language Association (MLA)
Ha, Xiao-qin…[et al.]. Inhibitory Effects of the Attenuated Salmonella typhimurium Containing the IL-2 Gene on Hepatic Tumors in Mice. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991963
American Medical Association (AMA)
Ha, Xiao-qin& Yin, Qiang& Zhao, Hong-bin& Hui, Ling& Wang, Mei-liang& Peng, Jun-hua…[et al.]. Inhibitory Effects of the Attenuated Salmonella typhimurium Containing the IL-2 Gene on Hepatic Tumors in Mice. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991963
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991963