Cytotoxicity of Bacterial Metabolic Products, including Listeriolysin O, on Leukocyte Targets
Joint Authors
Roeske, K.
Kawiak, J.
Lyzniak, M.
Budziszewska, B. K.
Stachowiak, R.
Hoser, G.
Bielecki, Jacek
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-10-01
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Bacterial toxins can exhibit anticancer activities.
Here we investigated the anticancer effects of the listeriolysin O toxin produced by Listeria monocytogenes.
We found that supernatants of Listeria monocytogenes strains (wild type, 1189, and 1190) were cytotoxic to the Jurkat cell line and human peripheral blood mononuclear cells (PBMC) in a concentration-dependent manner.
The supernatant of strain 1044, not producing listeriolysin O, was inactive.
The supernatants of Listeria strains were also cytotoxic toward B cells of chronic leukemia patients, with no significant differences in activities between strains.
We also tested supernatants of Bacillus subtilis strains BR1-90, BR1-S, and BR1-89 producing listeriolysin O.
BR1-S and BR1-89 were cytotoxic to PBMC and to Jurkat cells, the latter being more sensitive to the supernatants.
BR1-90 was not hemolytic or cytotoxic to PBMC, but was cytotoxic to Jurkat cells in the concentration range of 10–30%, suggesting that listeriolysin O is selectively effective against T cells.
Overall, the response of human peripheral blood mononuclear and human leukemia cell lines to bacteria supernatants containing listeriolysin O depended on the bacteria strain, target cell type, and supernatant concentration.
American Psychological Association (APA)
Stachowiak, R.& Lyzniak, M.& Budziszewska, B. K.& Roeske, K.& Bielecki, Jacek& Hoser, G.…[et al.]. 2012. Cytotoxicity of Bacterial Metabolic Products, including Listeriolysin O, on Leukocyte Targets. BioMed Research International،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-991970
Modern Language Association (MLA)
Stachowiak, R.…[et al.]. Cytotoxicity of Bacterial Metabolic Products, including Listeriolysin O, on Leukocyte Targets. BioMed Research International No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-991970
American Medical Association (AMA)
Stachowiak, R.& Lyzniak, M.& Budziszewska, B. K.& Roeske, K.& Bielecki, Jacek& Hoser, G.…[et al.]. Cytotoxicity of Bacterial Metabolic Products, including Listeriolysin O, on Leukocyte Targets. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-991970
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991970