Localization and Regulation of the N Terminal Splice Variant of PGC-1α in Adult Skeletal Muscle Fibers
Joint Authors
Shen, Tiansheng
Liu, Yewei
Schneider, Martin F.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-29
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The transcriptional coactivator peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α) regulates expression of genes for metabolism and muscle fiber type.
Recently, a novel splice variant of PGC-1α (NT-PGC-1α, amino acids 1–270) was cloned and found to be expressed in muscle.
Here we use Flag-tagged NT-PGC-1α to examine the subcellular localization and regulation of NT-PGC-1α in skeletal muscle fibers.
Flag-NT-PGC-1α is located predominantly in the myoplasm.
Nuclear NT-PGC-1α can be increased by activation of protein kinase A.
Activation of p38 MAPK by muscle activity or of AMPK had no effect on the subcellular distribution of NT-PGC-1α.
Inhibition of CRM1-mediated export only caused relatively slow nuclear accumulation of NT-PGC-1α, indicating that nuclear export of NT-PGC-1α may be mediated by both CRM1-dependent and -independent pathways.
Together these results suggest that the regulation of NT-PGC-1α in muscle fibers may be very different from that of the full-length PGC-1α, which is exclusively nuclear.
American Psychological Association (APA)
Shen, Tiansheng& Liu, Yewei& Schneider, Martin F.. 2012. Localization and Regulation of the N Terminal Splice Variant of PGC-1α in Adult Skeletal Muscle Fibers. BioMed Research International،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991990
Modern Language Association (MLA)
Shen, Tiansheng…[et al.]. Localization and Regulation of the N Terminal Splice Variant of PGC-1α in Adult Skeletal Muscle Fibers. BioMed Research International No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991990
American Medical Association (AMA)
Shen, Tiansheng& Liu, Yewei& Schneider, Martin F.. Localization and Regulation of the N Terminal Splice Variant of PGC-1α in Adult Skeletal Muscle Fibers. BioMed Research International. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991990
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991990