Validation of the Antiproliferative Effects of Organic Extracts from the Green Husk of Juglans regia L. on PC-3 Human Prostate Cancer Cells by Assessment of Apoptosis-Related Genes
Joint Authors
Alshatwi, Ali A.
Hasan, Tarique N.
Shafi, Gowhar
Syed, Naveed Ahmed
Al-Assaf, Abdullah H.
Alamri, Mohammed S.
Al-Khalifa, Abdrohman S.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
With the increased use of plant-based cancer chemotherapy, exploring the antiproliferative effects of phytochemicals for anticancer drug design has gained considerable attention worldwide.
This study was undertaken to investigate the effect of walnut green husk extracts on cell proliferation and to determine the possible molecular mechanism of extract-induced cell death by quantifying the expression of Bcl-2, Bax, caspases-3, and Tp53.
PC-3 human prostate cancer cells.
In this study, we found that green husk extracts suppressed proliferation and induced apoptosis in a dose- and time-dependent manner by modulating expression of apoptosis-related genes.
This involved DNA fragmentation (determined by TUNEL assay) and significant changes in levels of mRNA and the expression of corresponding proteins.
An increase in expressions of Bax, caspase-3, and tp53 genes and their corresponding proteins was detected using real-time PCR and western blot analysis in PC-3 cells treated with the green husk organic extracts.
In contrast, Bcl2 expression was downregulated after exposure to the extracts.
Our data suggest the presence of bioactive compound(s) in walnut green husks that are capable of killing prostate carcinoma cells by inducing apoptosis and that the husks are a candidate source of anticancer drugs.
American Psychological Association (APA)
Alshatwi, Ali A.& Hasan, Tarique N.& Shafi, Gowhar& Syed, Naveed Ahmed& Al-Assaf, Abdullah H.& Alamri, Mohammed S.…[et al.]. 2012. Validation of the Antiproliferative Effects of Organic Extracts from the Green Husk of Juglans regia L. on PC-3 Human Prostate Cancer Cells by Assessment of Apoptosis-Related Genes. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991997
Modern Language Association (MLA)
Alshatwi, Ali A.…[et al.]. Validation of the Antiproliferative Effects of Organic Extracts from the Green Husk of Juglans regia L. on PC-3 Human Prostate Cancer Cells by Assessment of Apoptosis-Related Genes. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-991997
American Medical Association (AMA)
Alshatwi, Ali A.& Hasan, Tarique N.& Shafi, Gowhar& Syed, Naveed Ahmed& Al-Assaf, Abdullah H.& Alamri, Mohammed S.…[et al.]. Validation of the Antiproliferative Effects of Organic Extracts from the Green Husk of Juglans regia L. on PC-3 Human Prostate Cancer Cells by Assessment of Apoptosis-Related Genes. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-991997
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-991997