Effect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice
Joint Authors
Nath, Debjani
Saha, S.
Mukhopadhyay, M. K.
Ghosh, P. D.
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The aim of the present study was to investigate the protective role of methanolic leaf extract of Ocimum basilicum L.
against benzene-induced hematotoxicity in Swiss albino mice.
GC analysis and subacute toxicity level of the extract were tested.
Mice were randomly divided into three groups among which II and III were exposed to benzene vapour at a dose 300 ppm × 6 hr/day × 5 days/week for 2 weeks and group I was control.
Group III of this experiment was treated with the leaf methanolic extract at a dose of 100 mg/kg body weight, a dose in nontoxic range.
Hematological parameters (Hb%, RBC and WBC counts), cell cycle regulatory proteins expression and DNA fragmentation analysis of bone marrow cells was performed.
There was an upregulation of p53 and p21 and downregulation of levels of CDK2, CDK4, CDK6, and cyclins D1 and E in leaf extract-treated group.
DNA was less fragmented in group III compared to group II (P<0.05).
The present study indicates that the secondary metabolites of O.
basilicum L.
methanolic leaf extract, comprising essential oil monoterpene geraniol and its oxidized form citral as major constituents, have modulatory effect in cell cycle deregulation and hematological abnormalities induced by benzene in mice.
American Psychological Association (APA)
Saha, S.& Mukhopadhyay, M. K.& Ghosh, P. D.& Nath, Debjani. 2012. Effect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-992083
Modern Language Association (MLA)
Saha, S.…[et al.]. Effect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-992083
American Medical Association (AMA)
Saha, S.& Mukhopadhyay, M. K.& Ghosh, P. D.& Nath, Debjani. Effect of Methanolic Leaf Extract of Ocimum basilicum L. on Benzene-Induced Hematotoxicity in Mice. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-992083
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992083