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Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats
Joint Authors
Choudhary, Shailesh Kumar
Chhabra, Gagan
Sharma, Dipali
Vashishta, Aruna
Ohri, Sujata
Dixit, Aparna
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-20
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The present study evaluates anti-hyperglycemic activity of fractionated Momordica charantia (bitter gourd) seed extracts.
Fasting blood glucose levels were evaluated before and after administration of different fractions of the seed extract.
Among the three fractions tested, fraction Mc-3 (15 mg/kg b.wt.) showed the maximum anti-hyperglycemic activity and reduced blood glucose levels in experimental diabetic rats significantly.
The activities of the key regulatory enzymes of glucose metabolism (hexokinase, pyruvate kinase, lactate dehydrogenase, and glucose-6-phosphate dehydrogenase) were determined in Mc-3-treated diabetic animals.
Once-daily administration of the fraction Mc-3 for prolonged period of 18 days to the experimental diabetic animals did not result in any nephrotoxicity or hepatotoxicity as evident from insignificant changes in biochemical parameters indicative of liver and kidney functions.
Further fractionation of the fraction Mc-3 by size exclusion chromatography resulted in a fraction, designated Mc-3.2, possessing anti-hyperglycemic activity.
The fraction Mc-3.2 showed the presence of a predominant protein band of ~11 kDa on SDS-PAGE.
Loss in anti-hyperglycemic activity of the Mc-3.2 upon protease treatment indicates the proteinaceous nature of the anti-hyperglycemic principles.
Overall, the results suggest that Momordica charantia seeds contain an effective anti-hyperglycemic protein(s) which may find application in treatment of diabetes without evident toxic effects.
American Psychological Association (APA)
Choudhary, Shailesh Kumar& Chhabra, Gagan& Sharma, Dipali& Vashishta, Aruna& Ohri, Sujata& Dixit, Aparna. 2012. Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-992174
Modern Language Association (MLA)
Choudhary, Shailesh Kumar…[et al.]. Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-992174
American Medical Association (AMA)
Choudhary, Shailesh Kumar& Chhabra, Gagan& Sharma, Dipali& Vashishta, Aruna& Ohri, Sujata& Dixit, Aparna. Comprehensive Evaluation of Anti-hyperglycemic Activity of Fractionated Momordica charantia Seed Extract in Alloxan-Induced Diabetic Rats. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-992174
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992174