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Anti-Hepatitis B Virus Effect and Possible Mechanism of Action of 3,4-O-Dicaffeoylquinic Acid In Vitro and In Vivo
Joint Authors
Wu, Yi-Hang
Hao, Bing-Jie
Cao, Hong-Cui
Xu, Wei
Li, Yong-Jun
Li, Lanjuan
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-03
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The anti-hepatitis B activity of 3,4-O-dicaffeoylquinic acid isolated from Laggera alata was studied using the d-galactosamine- (d-GalN-) induced hepatocyte damage model, HepG2.2.15 cells, and with HBV transgenic mice.
In vitro results showed that 3,4-O-dicaffeoylquinic acid improved HL-7702 hepatocyte viability and markedly inhibited the production of HBsAg and HBeAg.
At a concentration of 100 μg/mL, its inhibitory rates on the expression levels of HBsAg and HBeAg were 89.96% and 81.01%, respectively.
The content of hepatitis B virus covalently closed circular DNA (HBV cccDNA) in HepG2.2.15 cells was significantly decreased after the cells were treated with the test compound.
In addition, 3,4-O-dicaffeoylquinic acid significantly increased the expression of heme oxygenase-1 (HO-1) in HepG2.2.15 cells.
In vivo results indicated that the test compound at concentrations of 100 μg/mL significantly inhibited HBsAg production and increased HO-1 expression in HBV transgenic mice.
In conclusion, this study verifies the anti-hepatitis B activity of 3,4-O-dicaffeoylquinic acid.
The upregulation of HO-1 may contribute to the anti-HBV effect of this compound by reducing the stability of the HBV core protein, which blocks the refill of nuclear HBV cccDNA.
Furthermore, the hepatoprotective effect of this compound may be mediated through its antioxidative/anti-inflammatory properties and by the induction of HO-1 expression.
American Psychological Association (APA)
Wu, Yi-Hang& Hao, Bing-Jie& Cao, Hong-Cui& Xu, Wei& Li, Yong-Jun& Li, Lanjuan. 2012. Anti-Hepatitis B Virus Effect and Possible Mechanism of Action of 3,4-O-Dicaffeoylquinic Acid In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-992214
Modern Language Association (MLA)
Wu, Yi-Hang…[et al.]. Anti-Hepatitis B Virus Effect and Possible Mechanism of Action of 3,4-O-Dicaffeoylquinic Acid In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-992214
American Medical Association (AMA)
Wu, Yi-Hang& Hao, Bing-Jie& Cao, Hong-Cui& Xu, Wei& Li, Yong-Jun& Li, Lanjuan. Anti-Hepatitis B Virus Effect and Possible Mechanism of Action of 3,4-O-Dicaffeoylquinic Acid In Vitro and In Vivo. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-992214
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992214