Melanogenesis and Antityrosinase Activity of Selected South African Plants
Joint Authors
Mapunya, Manyatja Brenda
Nikolova, Roumiana Vassileva
Lall, Namrita
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-24
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Melanin is the pigment that is responsible for the colour of eyes, hair, and skin in humans.
Tyrosinase is known to be the key enzyme in melanin biosynthesis.
Overactivity of this enzyme leads to dermatological disorders such as age spots, melanoma and sites of actinic damage.
Ten plants belonging to four families (Asphodelaceae, Anacardiaceae, Oleaceae, and Rutaceae) were investigated for their effect on tyrosinase using both L-tyrosine and L-DOPA as substrates.
Ethanol leaf extracts (500 μg/mL) of Aloe ferox, Aloe aculeata, Aloe pretoriensis, and Aloe sessiliflora showed 60%, 31%, 17%, and 13% inhibition of tyrosinase activity respectively, when L-tyrosine was used as a substrate.
Harpephyllum caffrum (leaves) at a concentration of 500 μg/mL had an inhibitory effect of 70% on tyrosinase when L-DOPA was used as a substrate.
The IC50 of Harpephyllum caffrum (leaves and bark) were found to be 51±0.002 and 40±0.035 μg/mL, respectively.
Following the results obtained from the tyrosinase assay, extracts from Harpephyllum caffrum were selected for further testing on their effect on melanin production and their cytotoxicity on melanocytes in vitro.
The IC50 of both extracts was found to be 6.25 μg/mL for melanocyte cells.
Bark extract of Harpephyllum caffrum showed 26% reduction in melanin content of melanocyte cells at a concentration of 6.25 μg/mL.
The leaf extract of this plant showed some toxicity on melanocyte cells.
Therefore, the bark extract of Harpephyllum caffrum could be considered as an antityrosinase agent for dermatological disorders such as age spots and melasoma.
American Psychological Association (APA)
Mapunya, Manyatja Brenda& Nikolova, Roumiana Vassileva& Lall, Namrita. 2012. Melanogenesis and Antityrosinase Activity of Selected South African Plants. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-992230
Modern Language Association (MLA)
Mapunya, Manyatja Brenda…[et al.]. Melanogenesis and Antityrosinase Activity of Selected South African Plants. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-992230
American Medical Association (AMA)
Mapunya, Manyatja Brenda& Nikolova, Roumiana Vassileva& Lall, Namrita. Melanogenesis and Antityrosinase Activity of Selected South African Plants. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-992230
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992230