![](/images/graphics-bg.png)
Anticancer Activity of Certain Herbs and Spices on the Cervical Epithelial Carcinoma (HeLa) Cell Line
Joint Authors
Berrington, Danielle
Lall, Namrita
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-09
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Acetone extracts of selected plant species were evaluated for their in vitro cytotoxicity against a noncancerous African green monkey kidney (Vero) cell line and an adenocarcinoma cervical cancer (HeLa) cell line.
The plants studied were Origanum vulgare L.
(Oregano), Rosmarinus officinalis L.
(Upright and ground cove rosemary), Lavandula spica L.
(Lavender), Laurus nobilis L.
(Bay leaf), Thymus vulgaris L.
(Thyme), Lavandula x intermedia L.
(Margaret Roberts Lavender), Petroselinum crispum Mill.
(Curly leaved parsley), Foeniculum vulgare Mill.
(Fennel), and Capsicum annuum L.
(Paprika).
Antioxidant activity was determined using a quantitative DPPH (1,1-diphenyl-2-picryl hydrazyl) assay.
The rosemary species exhibited effective radical scavenging capacity with 50% inhibitory concentration (IC50) of 3.48±0.218 μg/mL and 10.84±0.125 μg/mL and vitamin C equivalents of 0.351 g and 1.09 g for McConnell’s Blue and Tuscan Blue, respectively.
Cytotoxicity was measured using XTT (Sodium 3′-[1-(phenyl amino-carbonyl)-3,4-tetrazolium]-bis-[4-methoxy-6-nitro] benzene sulfonic acid hydrate) colorimetric assay.
Only L.
nobilis and O.
vulgare exhibited pronounced effects on the HeLa cell line.
Dose-dependent studies revealed IC50 of 34.46±0.48 μg/mL and 126.3±1.00 μg/mL on the HeLa cells and on the Vero cells 124.1 μg/mL ± 18.26 and 163.8 μg/mL ± 2.95 for L.
nobilis and O.
vulgare, respectively.
Light (eosin and haematoxylin staining) and confocal microscopy (Hoechst 33342, acridine orange, and propidium iodide staining) were used to evaluate the cytotoxic mechanism of action for L.
nobilis and O.
vulgare.
American Psychological Association (APA)
Berrington, Danielle& Lall, Namrita. 2012. Anticancer Activity of Certain Herbs and Spices on the Cervical Epithelial Carcinoma (HeLa) Cell Line. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992411
Modern Language Association (MLA)
Berrington, Danielle& Lall, Namrita. Anticancer Activity of Certain Herbs and Spices on the Cervical Epithelial Carcinoma (HeLa) Cell Line. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-992411
American Medical Association (AMA)
Berrington, Danielle& Lall, Namrita. Anticancer Activity of Certain Herbs and Spices on the Cervical Epithelial Carcinoma (HeLa) Cell Line. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992411
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992411