Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated from Leea indica

Joint Authors

Yau Hsiung, Wong
Abdul Kadir, Habsah

Source

Evidence-Based Complementary and Alternative Medicine

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2011-11-20

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Medicine

Abstract EN

Leea indica is a medicinal plant traditionally used to treat cancer.

Through bioassay-guided approach, we isolated mollic acid arabinoside (MAA), for the first time from Leea indica.

Here, we present the apoptosis-inducing effect of MAA on Ca Ski cervical cancer cells.

Based on DAPI staining, MAA-treated cells manifested nuclear shrinkage, condensation, and fragmentation.

We further confirmed the fragmentation of DNA using TUNEL assay.

During early apoptosis, MAA caused the perturbation of plasma membrane through externalization of PS, followed by the formation of apoptotic blebs.

Prior to these events, MAA triggered rapid dissipation of the mitochondrial membrane potential.

In the upstream, MAA increased the expression of Bax, decreased the expression of Bcl-2, and augmented the Bax/Bcl-2 ratio.

These findings suggested that MAA induced mitochondrial-mediated apoptosis in Ca Ski cells and thus provide the scientific explanation for the traditional application of this herbal medicine in cancer treatment.

American Psychological Association (APA)

Yau Hsiung, Wong& Abdul Kadir, Habsah. 2011. Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated from Leea indica. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992533

Modern Language Association (MLA)

Yau Hsiung, Wong& Abdul Kadir, Habsah. Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated from Leea indica. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-992533

American Medical Association (AMA)

Yau Hsiung, Wong& Abdul Kadir, Habsah. Induction of Mitochondria-Mediated Apoptosis in Ca Ski Human Cervical Cancer Cells Triggered by Mollic Acid Arabinoside Isolated from Leea indica. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992533

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-992533