Reversion of P-Glycoprotein-Mediated Multidrug Resistance in Human Leukemic Cell Line by Diallyl Trisulfide
Joint Authors
Xia, Qing
Wang, Zhi-Yong
Li, Hui-Qing
Diao, Yu-Tao
Li, Xiao-Li
Cui, Jia
Chen, Xue-Liang
Li, Hao
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-12
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Multidrug resistance (MDR) is the major obstacle in chemotherapy, which involves multiple signaling pathways.
Diallyl trisulfide (DATS) is the main sulfuric compound in garlic.
In the present study, we aimed to explore whether DATS could overcome P-glycoprotein-(P-gp-)mediated MDR in K562/A02 cells, and to investigate whether NF-κB suppression is involved in DATS-induced reversal of MDR.
MTT assay revealed that cotreatment with DATS increased the response of K562/A02 cells to adriamycin (the resistance reversal fold was 3.79) without toxic side effects.
DATS could enhance the intracellular concentration of adriamycin by inhibiting the function and expression of P-gp, as shown by flow cytometry, RT-PCR, and western blot.
In addition, DATS resulted in more K562/A02 cell apoptosis, accompanied by increased expression of caspase-3.
The expression of NF-κB/p65 (downregulation) was significantly linked to the drug-resistance mechanism of DATS, whereas the expression of IκBα was not affected by DATS.
Our findings demonstrated that DATS can serve as a novel, nontoxic modulator of MDR, and can reverse the MDR of K562/A02 cells in vitro by increasing intracellular adriamycin concentration and inducing apoptosis.
More importantly, we proved for the first time that the suppression of NF-κB possibly involves the molecular mechanism in the course of reversion by DATS.
American Psychological Association (APA)
Xia, Qing& Wang, Zhi-Yong& Li, Hui-Qing& Diao, Yu-Tao& Li, Xiao-Li& Cui, Jia…[et al.]. 2012. Reversion of P-Glycoprotein-Mediated Multidrug Resistance in Human Leukemic Cell Line by Diallyl Trisulfide. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992580
Modern Language Association (MLA)
Xia, Qing…[et al.]. Reversion of P-Glycoprotein-Mediated Multidrug Resistance in Human Leukemic Cell Line by Diallyl Trisulfide. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-992580
American Medical Association (AMA)
Xia, Qing& Wang, Zhi-Yong& Li, Hui-Qing& Diao, Yu-Tao& Li, Xiao-Li& Cui, Jia…[et al.]. Reversion of P-Glycoprotein-Mediated Multidrug Resistance in Human Leukemic Cell Line by Diallyl Trisulfide. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992580
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992580