Inhibitory Effect of Curcumol on Jak2-STAT Signal Pathway Molecules of Fibroblast-Like Synoviocytes in Patients with Rheumatoid Arthritis
Joint Authors
Wang, Heng
Fang, Yongfei
Wang, Yong
Wang, Zhizhong
Zou, Qinghua
Shi, Ying
Chen, Juerong
Peng, Daizhi
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-12
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Hyperplasia of synovial membrane in rheumatoid arthritis (RA) is a critical pathological foundation for inducing articular injury.
The janus kinase and signal transducer and activator of transcription (Jak-STAT) pathway plays a critical role in synovial membrane proliferation induced by platelet-derived growth factor (PDGF).
To explore the anti-cell proliferation mechanism of curcumol, a pure monomer extracted from Chinese medical plant zedoary rhizome, the changes of Jak2-STAT1/3 signal pathway-related molecules in synoviocytes were observed in vitro.
In this study, the fibroblast-like synoviocytes (FLS) in patients with RA were collected and cultured.
The following parameters were measured: cell proliferation (WST-1 assay), cell cycles (fluorescence-activated cell sorting, FACS), STAT1 and STAT3 activities (electrophoretic mobility shift assay, EMSA), and the protein expressions of phosphorylated Jak2, STAT1, and STAT3 (Western blot).
It was shown that curcumol could inhibit the RA-FLS proliferation and DNA synthesis induced by PDGF-BB in a dose-dependent manner in vitro.
The transcription factors activities of STAT1 and STAT3 were obviously elevated after PDGF-BB stimulation (P<0.05).
Super-shift experiments identified the STAT1 or STAT3 proteins in the complex.
Furthermore, the different concentration curcumol could downregulate the DNA binding activities of STAT1 and STAT3 (P<0.05) and inhibit the phosphorylation of Jak2 while it had no effect on the protein expressions of STAT1 and STAT3.
Positive correlations were found between changes of cell proliferation and DNA-binding activities of STAT1 and STAT3, respectively (P<0.01).
In conclusion, curcumol might suppress the FLS proliferation and DNA synthesis induced by PDGF-BB through attenuating Jak2 phosphorylation, downregulating STAT1 and STAT3 DNA-binding activities, which could provide theoretical foundation for clinical treatment of RA.
American Psychological Association (APA)
Wang, Heng& Fang, Yongfei& Wang, Yong& Wang, Zhizhong& Zou, Qinghua& Shi, Ying…[et al.]. 2012. Inhibitory Effect of Curcumol on Jak2-STAT Signal Pathway Molecules of Fibroblast-Like Synoviocytes in Patients with Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992611
Modern Language Association (MLA)
Wang, Heng…[et al.]. Inhibitory Effect of Curcumol on Jak2-STAT Signal Pathway Molecules of Fibroblast-Like Synoviocytes in Patients with Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-992611
American Medical Association (AMA)
Wang, Heng& Fang, Yongfei& Wang, Yong& Wang, Zhizhong& Zou, Qinghua& Shi, Ying…[et al.]. Inhibitory Effect of Curcumol on Jak2-STAT Signal Pathway Molecules of Fibroblast-Like Synoviocytes in Patients with Rheumatoid Arthritis. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992611
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992611