Synergistic Antimycobacterial Actions of Knowltonia vesicatoria (L.f) Sims
Joint Authors
Labuschagné, Antoinette
Hussein, Ahmed A.
Rodríguez, Benjamín
Lall, Namrita
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-07
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Euclea natalensis A.DC., Knowltonia vesicatoria (L.f) Sims, and Pelargonium sidoides DC.
are South African plants traditionally used to treat tuberculosis.
Extracts from these plants were used in combination with isoniazid (INH) to investigate the possibility of synergy with respect to antimycobacterial activity.
The ethanol extract of K.
vesicatoria was subjected to fractionation to identify the active compounds.
The activity of the Knowltonia extract remained superior to the fractions with a minimum inhibitory concentration (MIC) of 625.0 μg/mL against Mycobacterium smegmatis and an MIC of 50.00 μg/mL against M.
tuberculosis.
The K.
vesicatoria extract was tested against two different drug-resistant strains of M.
tuberculosis, which resulted in an MIC of 50.00 μg/mL on both strains.
The combination of K.
vesicatoria with INH exhibited the best synergistic antimycobacterial activity with a fractional inhibitory concentration index of 0.25 (a combined concentration of 6.28 μg/mL).
A fifty percent inhibitory concentration of this combination against U937 cells was 121.0 μg/mL.
Two compounds, stigmasta-5,23-dien-3-ol (1) and 5-(hydroxymethyl)furan-2(5H)-one (2), were isolated from K.
vesicatoria as the first report of isolation for both compounds from this plant and the first report of antimycobacterial activity.
Compound (1) was active against drug-sensitive M.
tuberculosis with an MIC of 50.00 μg/mL.
American Psychological Association (APA)
Labuschagné, Antoinette& Hussein, Ahmed A.& Rodríguez, Benjamín& Lall, Namrita. 2012. Synergistic Antimycobacterial Actions of Knowltonia vesicatoria (L.f) Sims. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-992662
Modern Language Association (MLA)
Labuschagné, Antoinette…[et al.]. Synergistic Antimycobacterial Actions of Knowltonia vesicatoria (L.f) Sims. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-992662
American Medical Association (AMA)
Labuschagné, Antoinette& Hussein, Ahmed A.& Rodríguez, Benjamín& Lall, Namrita. Synergistic Antimycobacterial Actions of Knowltonia vesicatoria (L.f) Sims. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-992662
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992662