Active Hydrophilic Components of the Medicinal Herb Salvia miltiorrhiza (Danshen) Potently Inhibit Organic Anion Transporters 1 (Slc22a6) and 3 (Slc22a8)
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Many active components of herbal products are small organic anions, and organic anion transporters were previously demonstrated to be a potential site of drug-drug interactions.
In this study, we assessed the inhibitory effects of six hydrophilic components of the herbal medicine Danshen, lithospermic acid, protocatechuic acid, rosmarinic acid, salvianolic acid A, salvianolic acid B, and tanshinol, on the function of the murine organic anion transporters, mOat1 and mOat3.
All of Danshen components significantly inhibited mOat1- and mOat3-mediated substrate uptake (P<0.001) with lithospermic acid (LSA), protocatechuic acid, rosmarinic acid (RMA), and salvianolic acid A (SAA) producing virtually complete inhibition under test conditions.
Kinetic analysis demonstrated that LSA, RMA, and SAA were competitive inhibitors.
As such, Ki values were estimated as 14.9±4.9 μM for LSA, 5.5±2.2 μM for RMA, and 4.9±2.2 μM for SAA on mOat1-mediated transport, and as 31.1±7.0 μM for LSA, 4.3±0.2 μM for RMA, and 21.3±7.7 μM for SAA on mOat3-mediated transport.
These data suggest that herb-drug interactions may occur in vivo on the human orthologs of these transporters in situations of polypharmacy involving Danshen and clinical therapeutics known to be organic anion transporter substrates.
American Psychological Association (APA)
Wang, Li& Sweet, Douglas H.. 2012. Active Hydrophilic Components of the Medicinal Herb Salvia miltiorrhiza (Danshen) Potently Inhibit Organic Anion Transporters 1 (Slc22a6) and 3 (Slc22a8). Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992733
Modern Language Association (MLA)
Wang, Li& Sweet, Douglas H.. Active Hydrophilic Components of the Medicinal Herb Salvia miltiorrhiza (Danshen) Potently Inhibit Organic Anion Transporters 1 (Slc22a6) and 3 (Slc22a8). Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-992733
American Medical Association (AMA)
Wang, Li& Sweet, Douglas H.. Active Hydrophilic Components of the Medicinal Herb Salvia miltiorrhiza (Danshen) Potently Inhibit Organic Anion Transporters 1 (Slc22a6) and 3 (Slc22a8). Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992733
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992733