![](/images/graphics-bg.png)
Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism
Joint Authors
Chang, Yuan-Shiun
Chi, Chin-Wen
Lu, Kuan-Hung
Lee, Hui-Ju
Huang, Min-Li
Lai, Shang-Chih
Ho, Yu-Ling
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-07-27
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
The objective of this study was to examine the potential of enhancing the antileukemic activity of arsenic trioxide (ATO) by combining it with a folk remedy, crude methanolic extract of Mucuna macrocarpa (CMEMM).
Human leukemia cells HL-60, Jurkat, and Molt-3 were treated with various doses of ATO, CMEMM, and combinations thereof for 24 and 48 h.
Results indicated that the combination of 2.5 μM ATO and 50 μg/mL CMEMM synergistically inhibited cell proliferation in HL-60 and Jurkat cell lines.
Apoptosis triggered by ATO/CMEMM treatment was confirmed by accumulation of cells in the sub-G1 phase in cell cycle analyses, characteristic apoptotic nuclear fragmentation, and increased percentage of annexin V-positive apoptotic cells.
Such combination treatments also led to elevation of reactive oxygen species (ROS).
The antioxidants N-acetyl cysteine (NAC), butylated hydroxytoluene, and α-tocopherol prevented cells from ATO/CMEMM-induced apoptosis.
The ATO/CMEMM-induced activation of caspase-3 and caspase-9 can be blocked by NAC.
In summary, these results suggest that ATO/CMEMM combination treatment exerts synergistic apoptosis-inducing effects in human leukemic cells through a ROS-dependent mechanism and may provide a promising antileukemic approach in the future.
American Psychological Association (APA)
Lu, Kuan-Hung& Lee, Hui-Ju& Huang, Min-Li& Lai, Shang-Chih& Ho, Yu-Ling& Chang, Yuan-Shiun…[et al.]. 2011. Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-992771
Modern Language Association (MLA)
Lu, Kuan-Hung…[et al.]. Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-14.
https://search.emarefa.net/detail/BIM-992771
American Medical Association (AMA)
Lu, Kuan-Hung& Lee, Hui-Ju& Huang, Min-Li& Lai, Shang-Chih& Ho, Yu-Ling& Chang, Yuan-Shiun…[et al.]. Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-14.
https://search.emarefa.net/detail/BIM-992771
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992771