Viscum album L. Extracts Protects HeLa Cells against Nuclear and Mitochondrial DNA Damage
Joint Authors
Önay-Uçar, Evren
Erol, Özlem
Kandemir, Başak
Mertoğlu, Elif
Karagöz, Ali
Arda, Nazlı
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-04
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Viscum album L.
is a semiparasitic plant grown on trees and widely used for the treatment of many diseases in traditional and complementary therapy.
It is well known that some activities of Viscum album extracts are varied depending on the host trees, such as antioxidant, apoptosis-inducing, anticancer activities of the plant.
The aim of the present study is to examine the comparative effects of methanolic extracts of V.
album grown on three different host trees (locust tree, lime tree, and hedge maple tree) on H2O2-induced DNA damage in HeLa cells.
Oxidative damage in mitochondrial DNA and two nuclear regions was assessed by QPCR assay.
The cells were pretreated with methanolic extracts (10 μg/mL) for 48 h, followed by the treatment with 750 μM H2O2 for 1 hour.
DNA damage was significantly induced by H2O2 while it was inhibited by V.
album extracts.
All extracts completely protected against nuclear DNA damage.
While the extract from lime tree or white locust tree entirely inhibited mitochondrial DNA damage, that from hedge maple tree inhibited by only 50%.
These results suggest that methanolic extracts of V.
album can prevent oxidative DNA damage, and the activity is dependent on the host tree.
American Psychological Association (APA)
Önay-Uçar, Evren& Erol, Özlem& Kandemir, Başak& Mertoğlu, Elif& Karagöz, Ali& Arda, Nazlı. 2012. Viscum album L. Extracts Protects HeLa Cells against Nuclear and Mitochondrial DNA Damage. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-992822
Modern Language Association (MLA)
Önay-Uçar, Evren…[et al.]. Viscum album L. Extracts Protects HeLa Cells against Nuclear and Mitochondrial DNA Damage. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-992822
American Medical Association (AMA)
Önay-Uçar, Evren& Erol, Özlem& Kandemir, Başak& Mertoğlu, Elif& Karagöz, Ali& Arda, Nazlı. Viscum album L. Extracts Protects HeLa Cells against Nuclear and Mitochondrial DNA Damage. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-992822
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992822