Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF-β1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways
Joint Authors
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2011-08-07
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Salvianolic acid B (SA-B) is water-soluble component of Radix Salvia miltiorrhiza.
The previous work indicated that SA-B can inhibit MAPK and Smad signaling in activated hepatic stellate cells (HSCs) to perform anti-fibrotic activity Lv et al.
2010.
However, some studies have shown that there is cross-talk between MAPK and Smad in certain cell types.
Thus, the anti-fibrotic action of SA-B may be through the cross-talk.
In order to clarify the mechanism of SA-B further, we knocked down Smad in LX-2 cells (SRV4) via RNAi, and then added TGF-β1, and PD98059 or SB203580 and SA-B.
The levels of p-MEK and p-p38 were inhibited by SA-B in SRV4 independent of TGF-β1.
The expression of Col I and α-SMA in SRV4 could be reduced by SA-B independent TGF-β1.
SB203580 had not significant effect on p-MEK in SRV4 stimulated by TGF-β1.
The levels of p-MEK in SRV4 were not increased significantly after TGF-β1 stimulation.
PD98059 had no effect on the levels of p-p38 in SRV4 irrespective of TGF-β1.
In conclusion, SA-B inhibits the synthesis of Col I in LX-2 cells independent of TGF-β1 stimulation, and the anti-fibrotic effect of SA-B is due to direct inhibition of p38 signaling and inhibition the cross-talk of Smad to ERK signaling.
American Psychological Association (APA)
Lv, Zhigang& Xu, Lie-Ming. 2011. Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF-β1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992824
Modern Language Association (MLA)
Lv, Zhigang& Xu, Lie-Ming. Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF-β1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-11.
https://search.emarefa.net/detail/BIM-992824
American Medical Association (AMA)
Lv, Zhigang& Xu, Lie-Ming. Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF-β1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways. Evidence-Based Complementary and Alternative Medicine. 2011. Vol. 2012, no. 2012, pp.1-11.
https://search.emarefa.net/detail/BIM-992824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992824