Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway
Joint Authors
Kim, Dae Sung
Jeon, Byoung Kook
Lee, Young Eun
Woo, Won Hong
Mun, Yeun Ja
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Diosgenin, a naturally occurring steroid saponin found abundantly in legumes and yams, is a precursor of various synthetic steroidal drugs.
Diosgenin is studied for the mechanism of its action in apoptotic pathway in human hepatocellular carcinoma cells.
Based on DAPI staining, diosgenin-treated cells manifested nuclear shrinkage, condensation, and fragmentation.
Treatment of HepG2 cells with 40 μM diosgenin resulted in activation of the caspase-3, -8, -9 and cleavage of poly-ADP-ribose polymerase (PARP) and the release of cytochrome c.
In the upstream, diosgenin increased the expression of Bax, decreased the expression of Bid and Bcl-2, and augmented the Bax/Bcl-2 ratio.
Diosgenin-induced, dose-dependent induction of apoptosis was accompanied by sustained phosphorylation of JNK, p38 MAPK and apoptosis signal-regulating kinase (ASK)-1, as well as generation of the ROS.
NAC administration, a scavenger of ROS, reversed diosgene-induced cell death.
These results suggest that diosgenin-induced apoptosis in HepG2 cells through Bcl-2 protein family-mediated mitochndria/caspase-3-dependent pathway.
Also, diosgenin strongly generated ROS and this oxidative stress might induce apoptosis through activation of ASK1, which are critical upstream signals for JNK/p38 MAPK activation in HepG2 cancer cells.
American Psychological Association (APA)
Kim, Dae Sung& Jeon, Byoung Kook& Lee, Young Eun& Woo, Won Hong& Mun, Yeun Ja. 2012. Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway. Evidence-Based Complementary and Alternative Medicine،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992850
Modern Language Association (MLA)
Kim, Dae Sung…[et al.]. Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway. Evidence-Based Complementary and Alternative Medicine No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-992850
American Medical Association (AMA)
Kim, Dae Sung& Jeon, Byoung Kook& Lee, Young Eun& Woo, Won Hong& Mun, Yeun Ja. Diosgenin Induces Apoptosis in HepG2 Cells through Generation of Reactive Oxygen Species and Mitochondrial Pathway. Evidence-Based Complementary and Alternative Medicine. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992850
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992850