Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies
Joint Authors
Birkhead, Tim
Ekblom, Robert
Horsburgh, Gavin J.
Slate, Jon
Burke, Terry
Source
International Journal of Genomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-01-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Next-generation sequencing of transcriptomes (RNA-Seq) is being used increasingly in studies of nonmodel organisms.
Here, we evaluate the effectiveness of normalising cDNA libraries prior to sequencing in a small-scale study of the zebra finch.
We find that assemblies produced from normalised libraries had a larger number of contigs but used fewer reads compared to unnormalised libraries.
Considerably more genes were also detected using the contigs produced from normalised cDNA, and microsatellite discovery was up to 73% more efficient in these.
There was a positive correlation between the detected expression level of genes in normalised and unnormalised cDNA, and there was no difference in the number of genes identified as being differentially expressed between blood and spleen for the normalised and unnormalised libraries.
We conclude that normalised cDNA libraries are preferable for many applications of RNA-Seq and that these can also be used in quantitative gene expression studies.
American Psychological Association (APA)
Ekblom, Robert& Slate, Jon& Horsburgh, Gavin J.& Birkhead, Tim& Burke, Terry. 2012. Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies. International Journal of Genomics،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992883
Modern Language Association (MLA)
Ekblom, Robert…[et al.]. Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies. International Journal of Genomics No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-992883
American Medical Association (AMA)
Ekblom, Robert& Slate, Jon& Horsburgh, Gavin J.& Birkhead, Tim& Burke, Terry. Comparison between Normalised and Unnormalised 454-Sequencing Libraries for Small-Scale RNA-Seq Studies. International Journal of Genomics. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992883
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992883