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Features of Methylation and Gene Expression in the Promoter-Associated CpG Islands Using Human Methylome Data
Joint Authors
Han, Leng
Du, Xin
Zhao, Zhongming
Guo, An-Yuan
Source
International Journal of Genomics
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-04
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
CpG islands are typically located in the 5′ end of genes and considered as gene markers because they play important roles in gene regulation via epigenetic change.
In this study, we compared the features of CpG islands identified by several major algorithms by setting the parameter cutoff values in order to obtain a similar number of CpG islands in a genome.
This approach allows us to systematically compare the methylation and gene expression patterns in the identified CpG islands.
We found that Takai and Jones’ algorithm tends to identify longer CpG islands but with weaker CpG island features (e.g., lower GC content and lower ratio of the observed over expected CpGs) and higher methylation level.
Conversely, the CpG clusters identified by Hackenberg et al.’s algorithm using stringent criteria are shorter and have stronger features and lower methylation level.
In addition, we used the genome-wide base-resolution methylation profile in two cell lines to show that genes with a lower methylation level at the promoter-associated CpG islands tend to express in more tissues and have stronger expression.
Our results validated that the DNA methylation of promoter-associated CpG islands suppresses gene expression at the genome level.
American Psychological Association (APA)
Du, Xin& Han, Leng& Guo, An-Yuan& Zhao, Zhongming. 2012. Features of Methylation and Gene Expression in the Promoter-Associated CpG Islands Using Human Methylome Data. International Journal of Genomics،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992908
Modern Language Association (MLA)
Du, Xin…[et al.]. Features of Methylation and Gene Expression in the Promoter-Associated CpG Islands Using Human Methylome Data. International Journal of Genomics No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-992908
American Medical Association (AMA)
Du, Xin& Han, Leng& Guo, An-Yuan& Zhao, Zhongming. Features of Methylation and Gene Expression in the Promoter-Associated CpG Islands Using Human Methylome Data. International Journal of Genomics. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-992908
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-992908