Amelioration of Glucolipotoxicity-Induced Endoplasmic Reticulum Stress by a “Chemical Chaperone” in Human THP-1 Monocytes
Joint Authors
Agrawal, Madhur
Balasubramanyam, Muthuswamy
Lenin, Raji
Mohan, Viswanathan
Maria, Mariawilliam Sneha
Balasubramanyam, Jayashree
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-10
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Chronic ER stress is emerging as a trigger that imbalances a number of systemic and arterial-wall factors and promote atherosclerosis.
Macrophage apoptosis within advanced atherosclerotic lesions is also known to increase the risk of atherothrombotic disease.
We hypothesize that glucolipotoxicity might mediate monocyte activation and apoptosis through ER stress.
Therefore, the aims of this study are (a) to investigate whether glucolipotoxicity could impose ER stress and apoptosis in THP-1 human monocytes and (b) to investigate whether 4-Phenyl butyric acid (PBA), a chemical chaperone could resist the glucolipotoxicity-induced ER stress and apoptosis.
Cells subjected to either glucolipotoxicity or tunicamycin exhibited increased ROS generation, gene and protein (PERK, GRP-78, IRE1α, and CHOP) expression of ER stress markers.
In addition, these cells showed increased TRPC-6 channel expression and apoptosis as revealed by DNA damage and increased caspase-3 activity.
While glucolipotoxicity/tunicamycin increased oxidative stress, ER stress, mRNA expression of TRPC-6, and programmed the THP-1 monocytes towards apoptosis, all these molecular perturbations were resisted by PBA.
Since ER stress is one of the underlying causes of monocyte dysfunction in diabetes and atherosclerosis, our study emphasize that chemical chaperones such as PBA could alleviate ER stress and have potential to become novel therapeutics.
American Psychological Association (APA)
Lenin, Raji& Maria, Mariawilliam Sneha& Agrawal, Madhur& Balasubramanyam, Jayashree& Mohan, Viswanathan& Balasubramanyam, Muthuswamy. 2012. Amelioration of Glucolipotoxicity-Induced Endoplasmic Reticulum Stress by a “Chemical Chaperone” in Human THP-1 Monocytes. Journal of Diabetes Research،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-993970
Modern Language Association (MLA)
Lenin, Raji…[et al.]. Amelioration of Glucolipotoxicity-Induced Endoplasmic Reticulum Stress by a “Chemical Chaperone” in Human THP-1 Monocytes. Journal of Diabetes Research Vol. 2012, no. 2012 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-993970
American Medical Association (AMA)
Lenin, Raji& Maria, Mariawilliam Sneha& Agrawal, Madhur& Balasubramanyam, Jayashree& Mohan, Viswanathan& Balasubramanyam, Muthuswamy. Amelioration of Glucolipotoxicity-Induced Endoplasmic Reticulum Stress by a “Chemical Chaperone” in Human THP-1 Monocytes. Journal of Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-993970
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-993970