Functional Expression of TRPV4 Channels in Human Collecting Duct Cells: Implications for Secondary Hypertension in Diabetic Nephropathy
Joint Authors
Squires, Paul E.
Bland, Rosemary
Hills, Claire E.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The Vanilloid subfamily of transient receptor potential (TRPV) ion channels has been widely implicated in detecting osmotic and mechanical stress.
In the current study, we examine the functional expression of TRPV4 channels in cell volume regulation in cells of the human collecting duct.
Methods.
Western blot analysis, siRNA knockdown, and microfluorimetry were used to assess the expression and function of TRPV4 in mediating Ca2+-dependent mechanical stimulation within a novel system of the human collecting duct (HCD).
Results.
Native and siRNA knockdown of TRPV4 protein expression was confirmed by western blot analysis.
Touch was used as a cell-directed surrogate for osmotic stress.
Mechanical stimulation of HCD cells evoked a transient increase in [Ca2+]i that was dependent upon thapsigargin-sensitive store release and Ca2+ influx.
At 48 hrs, high glucose and mannitol (25 mM) reduced TRPV4 expression by 54% and 24%, respectively.
Similar treatment doubled SGK1 expression.
Touch-evoked changes were negated following TRPV4 knockdown.
Conclusion.
Our data confirm expression of Ca2+-dependent TRPV4 channels in HCD cells and suggest that a loss of expression in response to high glucose attenuates the ability of the collecting duct to exhibit regulatory volume decreases, an effect that may contribute to the pathology of fluid and electrolyte imbalance as observed in diabetic nephropathy.
American Psychological Association (APA)
Hills, Claire E.& Bland, Rosemary& Squires, Paul E.. 2012. Functional Expression of TRPV4 Channels in Human Collecting Duct Cells: Implications for Secondary Hypertension in Diabetic Nephropathy. Journal of Diabetes Research،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-994128
Modern Language Association (MLA)
Hills, Claire E.…[et al.]. Functional Expression of TRPV4 Channels in Human Collecting Duct Cells: Implications for Secondary Hypertension in Diabetic Nephropathy. Journal of Diabetes Research Vol. 2012, no. 2012 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-994128
American Medical Association (AMA)
Hills, Claire E.& Bland, Rosemary& Squires, Paul E.. Functional Expression of TRPV4 Channels in Human Collecting Duct Cells: Implications for Secondary Hypertension in Diabetic Nephropathy. Journal of Diabetes Research. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-994128
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-994128