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Immune Responses to RHAMM in Patients with Acute Myeloid Leukemia after Chemotherapy and Allogeneic Stem Cell Transplantation
Joint Authors
Pützer, B. M.
Spitschak, A.
Casalegno-Garduño, R.
Schmitt, A.
Hirt, C.
Freund, M.
Rohde, S.
Schmitt, Michael
Hilgendorf, I.
Meier, C.
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-06-06
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Leukemic blasts overexpress immunogenic antigens, so-called leukemia-associated antigens like the receptor for hyaluronan acid-mediated motility (RHAMM).
Persistent RHAMM expression and decreasing CD8+ T-cell responses to RHAMM in the framework of allogeneic stem cell transplantation or chemotherapy alone might indicate the immune escape of leukemia cells.
In the present study, we analyzed the expression of RHAMM in 48 patients suffering from acute myeloid leukemia (AML) and myelodysplastic syndrome (MDS).
Furthermore, we correlated transcripts with the clinical course of the disease before and after treatment.
Real-time quantitative reverse transcriptase polymerase chain reaction was performed from RNA of peripheral blood mononuclear cells.
T cell responses against RHAMM were assessed by tetramer staining (flow cytometry) and enzyme-linked immunospot (ELISPOT) assays.
Results were correlated with the clinical outcome of patients.
The results of the present study showed that almost 60% of the patients were RHAMM positive; specific T-cells recognizing RHAMM could be detected, but they were nonfunctional in terms of interferon gamma or granzyme B release as demonstrated by ELISPOT assays.
Immunotherapies like peptide vaccination or adoptive transfer of RHAMM-specific T cells might improve the immune response and the outcome of AML/MDS patients.
American Psychological Association (APA)
Casalegno-Garduño, R.& Meier, C.& Schmitt, A.& Spitschak, A.& Hilgendorf, I.& Rohde, S.…[et al.]. 2012. Immune Responses to RHAMM in Patients with Acute Myeloid Leukemia after Chemotherapy and Allogeneic Stem Cell Transplantation. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-994341
Modern Language Association (MLA)
Casalegno-Garduño, R.…[et al.]. Immune Responses to RHAMM in Patients with Acute Myeloid Leukemia after Chemotherapy and Allogeneic Stem Cell Transplantation. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-9.
https://search.emarefa.net/detail/BIM-994341
American Medical Association (AMA)
Casalegno-Garduño, R.& Meier, C.& Schmitt, A.& Spitschak, A.& Hilgendorf, I.& Rohde, S.…[et al.]. Immune Responses to RHAMM in Patients with Acute Myeloid Leukemia after Chemotherapy and Allogeneic Stem Cell Transplantation. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-994341
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-994341