Gene Expression Profiling in Dermatitis Herpetiformis Skin Lesions
Joint Authors
Parodi, Aurora
Lunardi, C.
Puccetti, A.
Tinazzi, E.
Cozzani, Emanuele
Dolcino, M.
Riva, S.
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-06
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Dermatitis herpetiformis (DH) is an autoimmune blistering skin disease associated with gluten-sensitive enteropathy (CD).
In order to investigate the pathogenesis of skin lesions at molecular level, we analysed the gene expression profiles in skin biopsies from 6 CD patients with DH and 6 healthy controls using Affymetrix HG-U133A 2.0 arrays.
486 genes were differentially expressed in DH skin compared to normal skin: 225 were upregulated and 261 were downregulated.
Consistently with the autoimmune origin of DH, functional classification of the differentially expressed genes (DEGs) indicates a B- and T-cell immune response (LAG3, TRAF5, DPP4, and NT5E).
In addition, gene modulation provides evidence for a local inflammatory response (IL8, PTGFR, FSTL1, IFI16, BDKRD2, and NAMPT) with concomitant leukocyte recruitment (CCL5, ENPP2), endothelial cell activation, and neutrophil extravasation (SELL, SELE).
DEGs also indicate overproduction of matrix proteases (MMP9, ADAM9, and ADAM19) and proteolytic enzymes (CTSG, ELA2, CPA3, TPSB2, and CMA1) that may contribute to epidermal splitting and blister formation.
Finally, we observed modulation of genes involved in cell growth inhibition (CGREF1, PA2G4, and PPP2R1B), increased apoptosis (FAS, TNFSF10, and BASP1), and reduced adhesion at the dermal epidermal junction (PLEC1, ITGB4, and LAMA5).
In conclusion, our results identify genes that are involved in the pathogenesis of DH skin lesions.
American Psychological Association (APA)
Dolcino, M.& Cozzani, Emanuele& Riva, S.& Parodi, Aurora& Tinazzi, E.& Lunardi, C.…[et al.]. 2012. Gene Expression Profiling in Dermatitis Herpetiformis Skin Lesions. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-994367
Modern Language Association (MLA)
Dolcino, M.…[et al.]. Gene Expression Profiling in Dermatitis Herpetiformis Skin Lesions. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-994367
American Medical Association (AMA)
Dolcino, M.& Cozzani, Emanuele& Riva, S.& Parodi, Aurora& Tinazzi, E.& Lunardi, C.…[et al.]. Gene Expression Profiling in Dermatitis Herpetiformis Skin Lesions. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-994367
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-994367