Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2
Joint Authors
Wang, Yao
Xie, Guangyan
Li, Suqin
Pan, Mingjie
Li, Yuexi
Wang, Xingsheng
Zhang, Shumin
Liao, Jianmin
Pan, Ying
Yin, Dengke
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-05-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Twenty B candidate epitopes of glycoproteins B (gB2), C (gC2), E (gE2), G (gG2), and I (gI2) of herpes simplex virus type 2 (HSV-2) were predicted using DNAstar, Biosun, and Antheprot methods combined with the polynomial method.
Subsequently, the biological functions of the peptides were tested via experiments in vitro.
Among the 20 epitope peptides, 17 could react with the antisera to the corresponding parent proteins in the EIA tests.
In particular, five peptides, namely, gB2466–473 (EQDRKPRN), gC2216–223 (GRTDRPSA), gE2483–491 (DPPERPDSP), gG2572–579 (EPPDDDDS), and gI2286-295 (CRRRYRRPRG) had strong reaction with the antisera.
All conjugates of the five peptides with the carrier protein BSA could stimulate mice into producing antibodies.
The antisera to these peptides reacted strongly with the corresponding parent glycoproteins during the Western Blot tests, and the peptides reacted strongly with the antibodies against the parent glycoproteins during the EIA tests.
The antisera against the five peptides could neutralize HSV-2 infection in vitro, which has not been reported until now.
These results suggest that the immunodominant epitopes screened using software algorithms may be used for virus diagnosis and vaccine design against HSV-2.
American Psychological Association (APA)
Pan, Mingjie& Wang, Xingsheng& Liao, Jianmin& Yin, Dengke& Li, Suqin& Pan, Ying…[et al.]. 2012. Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-994368
Modern Language Association (MLA)
Pan, Mingjie…[et al.]. Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-8.
https://search.emarefa.net/detail/BIM-994368
American Medical Association (AMA)
Pan, Mingjie& Wang, Xingsheng& Liao, Jianmin& Yin, Dengke& Li, Suqin& Pan, Ying…[et al.]. Prediction and Identification of Potential Immunodominant Epitopes in Glycoproteins B, C, E, G, and I of Herpes Simplex Virus Type 2. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-994368
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-994368