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Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance
Joint Authors
Ruberti, Maristela
Tamashiro, Wirla Maria da Silva Cunha
Gabriel, Dirce Lima
Simioni, Patricia Ucelli
Fernandes, Luis Gustavo Romani
Yamada, Áureo Tatsumi
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-02-06
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
In this work, we evaluated the effects of administration of OVA on phenotype and function of intraepithelial lymphocytes (IELs) from small intestine of transgenic (TGN) DO11.10 and wild-type BALB/c mice.
While the small intestines from BALB/c presented a well preserved structure, those from TGN showed an inflamed aspect.
The ingestion of OVA induced a reduction in the number of IELs in small intestines of TGN, but it did not change the frequencies of CD8+ and CD4+ T-cell subsets.
Administration of OVA via oral + ip increased the frequency of CD103+ cells in CD4+ T-cell subset in IELs of both BALB/c and TGN mice and elevated its expression in CD8β+ T-cell subset in IELs of TGN.
The frequency of Foxp3+ cells increased in all subsets in IELs of BALB/c treated with OVA; in IELs of TGN, it increased only in CD25+ subset.
IELs from BALB/c tolerant mice had lower expression of all cytokines studied, whereas those from TGN showed high expression of inflammatory cytokines, especially of IFN-γ, TGF-β, and TNF-α.
Overall, our results suggest that the inability of TGN to become tolerant may be related to disorganization and altered proportions of inflammatory/regulatory T cells in its intestinal mucosa.
American Psychological Association (APA)
Ruberti, Maristela& Fernandes, Luis Gustavo Romani& Simioni, Patricia Ucelli& Gabriel, Dirce Lima& Yamada, Áureo Tatsumi& Tamashiro, Wirla Maria da Silva Cunha. 2012. Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-994371
Modern Language Association (MLA)
Ruberti, Maristela…[et al.]. Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-16.
https://search.emarefa.net/detail/BIM-994371
American Medical Association (AMA)
Ruberti, Maristela& Fernandes, Luis Gustavo Romani& Simioni, Patricia Ucelli& Gabriel, Dirce Lima& Yamada, Áureo Tatsumi& Tamashiro, Wirla Maria da Silva Cunha. Phenotypical and Functional Analysis of Intraepithelial Lymphocytes from Small Intestine of Mice in Oral Tolerance. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-16.
https://search.emarefa.net/detail/BIM-994371
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-994371