Variable EBV DNA Load Distributions and Heterogeneous EBV mRNA Expression Patterns in the Circulation of Solid Organ versus Stem Cell Transplant Recipients
Joint Authors
Verdonck, L. F.
Fleig, S. C.
Stevens, S. J.
Hepkema, B. G.
Cornelissen, J. J.
Verschuuren, E. A.
Greijer, A. E.
Brooimans, R. A.
Juwana, H.
Verkuijlen, S. A.
Middeldorp, J. M.
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-30
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Epstein-Barr virus (EBV) driven post-transplant lymphoproliferative disease (PTLD) is a heterogeneous and potentially life-threatening condition.
Early identification of aberrant EBV activity may prevent progression to B-cell lymphoma.
We measured EBV DNA load and RNA profiles in plasma and cellular blood compartments of stem cell transplant (SCT; n=5), solid organ transplant recipients (SOT; n=15), and SOT having chronic elevated EBV-DNA load (n=12).
In SCT, EBV DNA was heterogeneously distributed, either in plasma or leukocytes or both.
In SOT, EBV DNA load was always cell associated, predominantly in B cells, but occasionally in T cells (CD4 and CD8) or monocytes.
All SCT with cell-associated EBV DNA showed BARTs and EBNA1 expression, while LMP1 and LMP2 mRNA was found in 1 and 3 cases, respectively.
In SOT, expression of BARTs was detected in all leukocyte samples.
LMP2 and EBNA1 mRNA was found in 5/15 and 2/15, respectively, but LMP1 mRNA in only 1, coinciding with severe PTLD and high EBV DNA.
Conclusion: EBV DNA is differently distributed between white cells and plasma in SOT versus SCT.
EBV RNA profiling in blood is feasible and may have added value for understanding pathogenic virus activity in patients with elevated EBV-DNA.
American Psychological Association (APA)
Greijer, A. E.& Stevens, S. J.& Verkuijlen, S. A.& Juwana, H.& Fleig, S. C.& Verschuuren, E. A.…[et al.]. 2012. Variable EBV DNA Load Distributions and Heterogeneous EBV mRNA Expression Patterns in the Circulation of Solid Organ versus Stem Cell Transplant Recipients. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-994491
Modern Language Association (MLA)
Greijer, A. E.…[et al.]. Variable EBV DNA Load Distributions and Heterogeneous EBV mRNA Expression Patterns in the Circulation of Solid Organ versus Stem Cell Transplant Recipients. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-10.
https://search.emarefa.net/detail/BIM-994491
American Medical Association (AMA)
Greijer, A. E.& Stevens, S. J.& Verkuijlen, S. A.& Juwana, H.& Fleig, S. C.& Verschuuren, E. A.…[et al.]. Variable EBV DNA Load Distributions and Heterogeneous EBV mRNA Expression Patterns in the Circulation of Solid Organ versus Stem Cell Transplant Recipients. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-994491
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-994491