Automatic Reading of ANCA-Slides: Evaluation of the AKLIDES System
Joint Authors
Vaessen, Mia
Cohen Tervaert, Jan Willem
Austen, Jos
Damoiseaux, Jan
Mallet, Kathleen
Source
Journal of Immunology Research
Issue
Vol. 2012, Issue 2012 (31 Dec. 2011), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-11-05
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
The ANCA consensus prescribes screening by indirect immunofluorescence on neutrophils.
We evaluated the first automated ANCA-pattern recognition system.
C-ANCA (n=39) and P-ANCA (n=40) samples were selected from patients with ANCA-associated vasculitis (AAV).
Non-AAV controls included sera from healthy controls (n=40), sera with possible interfering antibodies (n=46), or miscellaneous ANCA reactivity (n=31).
ANCA slides were analysed by AKLIDES and routine fluorescence microscopy.
The C-ANCA pattern was recognized by routine microscopy in 92% and 97% on ethanol- and formalin-fixed slides, respectively.
AKLIDES reported C-ANCA in 74% and 95%, respectively.
P-ANCA was recognized by routine microscopy on ethanol-fixed neutrophils in 90%, while AKLIDES reported P-ANCA in 80%.
Typically, only 65% and 33% of these samples showed the expected C-ANCA on formalin-fixed neutrophils by routine microscopy and AKLIDES, respectively.
A C- or P-ANCA pattern was observed on ethanol-fixed neutrophils in 28% and 23% of the controls by routine microscopy and AKLIDES, respectively.
Only 5% of the controls revealed C-ANCA on formalin-fixed neutrophils by routine microscopy and AKLIDES.
Altogether, automated ANCA-pattern recognition by AKLIDES is promising.
Distinction of C- and P-ANCA is good, but sensitivity on ethanol-fixed neutrophils needs improvement.
When optimized, pattern recognition software may play an important role in AAV diagnostics.
American Psychological Association (APA)
Damoiseaux, Jan& Mallet, Kathleen& Vaessen, Mia& Austen, Jos& Cohen Tervaert, Jan Willem. 2012. Automatic Reading of ANCA-Slides: Evaluation of the AKLIDES System. Journal of Immunology Research،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-996819
Modern Language Association (MLA)
Damoiseaux, Jan…[et al.]. Automatic Reading of ANCA-Slides: Evaluation of the AKLIDES System. Journal of Immunology Research Vol. 2012, no. 2012 (2011), pp.1-7.
https://search.emarefa.net/detail/BIM-996819
American Medical Association (AMA)
Damoiseaux, Jan& Mallet, Kathleen& Vaessen, Mia& Austen, Jos& Cohen Tervaert, Jan Willem. Automatic Reading of ANCA-Slides: Evaluation of the AKLIDES System. Journal of Immunology Research. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-996819
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-996819