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Biphasic Osteogenic Characteristics of Human Mesenchymal Stem Cells Cultured on TiO2 Nanotubes of Different Diameters
Joint Authors
Moon, Kyung-Suk
Yu, Sang-Hui
Bae, Ji-Myung
Oh, Seunghan
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-09
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
We cultured human mesenchymal stem cells (hMSCs) on TiO2 nanotubes with diameters of 30–100 nm to assess the size-effect of TiO2 nanotubes on the behavior and osteogenic functionality of hMSCs.
Most studies of the expression of genes encoding alkaline phosphatase (ALP), osteocalcin (OCN), osteopontin (OPN), and integrin-β (INT-B), after 1 week of incubation, supported the results of cell viability and MTT assays at 48 hrs of plating.
However, after 2 weeks of incubation, expression of ALP, OPN, INT-B, and protein kinase R-like ER kinase (PERK) genes were significantly higher in cells cultured on 70 nm TiO2 nanotubes than that in cells cultured on other TiO2 nanotubes and Ti.
This biphasic osteogenic characteristic of hMSCs is supposed to relating to the nature of the hMSCs adhering to the substrate at the beginning of incubation, and the nanostructural stimulation caused by the topography of TiO2 nanotubes at a later stage of incubation.
The discovery of these biphasic characteristics of hMSCs cultured on different-sized TiO2 nanotubes may contribute to resolving the discrepant results relating to the size-effect of TiO2 nanotubes on the adhesion, proliferation, and functionality of cells.
American Psychological Association (APA)
Moon, Kyung-Suk& Yu, Sang-Hui& Bae, Ji-Myung& Oh, Seunghan. 2012. Biphasic Osteogenic Characteristics of Human Mesenchymal Stem Cells Cultured on TiO2 Nanotubes of Different Diameters. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-997478
Modern Language Association (MLA)
Moon, Kyung-Suk…[et al.]. Biphasic Osteogenic Characteristics of Human Mesenchymal Stem Cells Cultured on TiO2 Nanotubes of Different Diameters. Journal of Nanomaterials No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-997478
American Medical Association (AMA)
Moon, Kyung-Suk& Yu, Sang-Hui& Bae, Ji-Myung& Oh, Seunghan. Biphasic Osteogenic Characteristics of Human Mesenchymal Stem Cells Cultured on TiO2 Nanotubes of Different Diameters. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-997478
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-997478