Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-Enhanced Fluorescence Imaging
Joint Authors
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-12
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
A highly sensitive immunosensor based on a gold nanopatterned chip was developed for accurate determination of alpha-fetoprotein (AFP) via total internal refection fluorescence microscopy (TIRFM).
The surface of the gold nanopatterned chips was modified with dithiobis(succinimidyl propionate) and protein A/G for immobilization of the AFP antibody.
The immunoassay created a sandwich of antigen between the AFP antibody on the chip that was modified with protein A/G, and the secondary antibody, a monoclonal anti-human-AFP labeled with biotin (biotin-labeled anti-AFP).
AFP concentration was determined based on evanescent field fluorescence signal, which was generated by interaction between biotin-labeled anti-AFP and a streptavidin-labeled fluorescence dye.
AFP concentration could be measured in a wide dynamic linear range of 720 zM–10 nM with a detection limit of 720 zM.
A significant enhanced sensitivity (~40,000-fold) was achieved with the AFP-nanoarray chip compared to conventional chemiluminescence immunosensors.
The immunoassay exhibited a wide detection range and high sensitivity and accuracy, qualities valuable for clinical assay of AFP.
American Psychological Association (APA)
Lee, Seungah& Kang, Seong Ho. 2012. Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-Enhanced Fluorescence Imaging. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-998254
Modern Language Association (MLA)
Lee, Seungah& Kang, Seong Ho. Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-Enhanced Fluorescence Imaging. Journal of Nanomaterials No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-998254
American Medical Association (AMA)
Lee, Seungah& Kang, Seong Ho. Single-Molecule Sandwich Immunoassay for Quantification of Alpha-Fetoprotein Based on Evanescent Field-Enhanced Fluorescence Imaging. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-998254
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998254