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Study of Cytotoxic and Genotoxic Effects of Hydroxyl-Functionalized Multiwalled Carbon Nanotubes on Human Pulmonary Cells
Joint Authors
Ursini, Cinzia Lucia
Cavallo, Delia
Fresegna, Anna Maria
Ciervo, Aureliano
Maiello, Raffaele
Casciardi, Stefano
Tombolini, Francesca
Buresti, Giuliana
Iavicoli, Sergio
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-02
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Chemical functionalization of multiwalled carbon nanotubes (MWCNTs) increases their solubility, dispersion, and biological applications.
Since there are only a few studies on the toxicity of functionalized MWCNTs, we investigated the cytotoxic and genotoxic-oxidative effects of OH-functionalized MWCNTs on human lung epithelial cells (A549) in order to obtain information on their biological effects.
We exposed the cells to 10, 20, 40, and 100 μg/mL of commercial MWCNT-OH for 24 h.
Cytotoxicity was then evaluated as the reduction in cell viability, membrane damage, and apoptosis, assessed by MTT and LDH assays and fluorescence microscopic analysis, respectively.
The Fpg-modified comet assay was used to assess direct/oxidative DNA damage.
We found a concentration-dependent reduction in cell viability and an increase of percentage of apoptotic cells, with no significant cellular LDH release.
There was also concentration-dependent direct DNA damage but no oxidative DNA damage.
These findings demonstrate the cytotoxicity of MWCNT-OH, through reduction of cell viability and induction of apoptosis without cell membrane damage, and the genotoxicity, by direct DNA damage induction, suggesting that the MWCNTs enter the cell without damaging its membrane and directly interact with the nucleus.
This preliminary study highlights the need for further research to examine the potential toxicity of functionalized MWCNTs before starting to use them in biological applications.
American Psychological Association (APA)
Ursini, Cinzia Lucia& Cavallo, Delia& Fresegna, Anna Maria& Ciervo, Aureliano& Maiello, Raffaele& Casciardi, Stefano…[et al.]. 2012. Study of Cytotoxic and Genotoxic Effects of Hydroxyl-Functionalized Multiwalled Carbon Nanotubes on Human Pulmonary Cells. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-998467
Modern Language Association (MLA)
Ursini, Cinzia Lucia…[et al.]. Study of Cytotoxic and Genotoxic Effects of Hydroxyl-Functionalized Multiwalled Carbon Nanotubes on Human Pulmonary Cells. Journal of Nanomaterials No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-998467
American Medical Association (AMA)
Ursini, Cinzia Lucia& Cavallo, Delia& Fresegna, Anna Maria& Ciervo, Aureliano& Maiello, Raffaele& Casciardi, Stefano…[et al.]. Study of Cytotoxic and Genotoxic Effects of Hydroxyl-Functionalized Multiwalled Carbon Nanotubes on Human Pulmonary Cells. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-998467
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998467