Nanostructured Surfaces to Target and Kill Circulating Tumor Cells While Repelling Leukocytes
Joint Authors
Mitchell, Michael J.
Castellanos, Carlos A.
King, Michael R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-12-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Hematogenous metastasis, the process of cancer cell migration from a primary to distal location via the bloodstream, typically leads to a poor patient prognosis.
Selectin proteins hold promise in delivering drug-containing nanocarriers to circulating tumor cells (CTCs) in the bloodstream, due to their rapid, force-dependent binding kinetics.
However, it is challenging to deliver such nanocarriers while avoiding toxic effects on healthy blood cells, as many possess ligands that adhesively interact with selectins.
Herein, we describe a nanostructured surface to capture flowing cancer cells, while preventing human neutrophil adhesion.
Microtube surfaces with immobilized halloysite nanotubes (HNTs) and E-selectin functionalized liposomal doxorubicin (ES-PEG L-DXR) significantly increased the number of breast adenocarcinoma MCF7 cells captured from flow, yet also significantly reduced the number of captured neutrophils.
Neutrophils firmly adhered and projected pseudopods on surfaces coated only with liposomes, while neutrophils adherent to HNT-liposome surfaces maintained a round morphology.
Perfusion of both MCF7 cells and neutrophils resulted in primarily cancer cell adhesion to the HNT-liposome surface, and induced significant cancer cell death.
This work demonstrates that nanostructured surfaces consisting of HNTs and ES-PEG L-DXR can increase CTC recruitment for chemotherapeutic delivery, while also preventing healthy cell adhesion and uptake of therapeutic intended for CTCs.
American Psychological Association (APA)
Mitchell, Michael J.& Castellanos, Carlos A.& King, Michael R.. 2012. Nanostructured Surfaces to Target and Kill Circulating Tumor Cells While Repelling Leukocytes. Journal of Nanomaterials،Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-998507
Modern Language Association (MLA)
Mitchell, Michael J.…[et al.]. Nanostructured Surfaces to Target and Kill Circulating Tumor Cells While Repelling Leukocytes. Journal of Nanomaterials No. 2012 (2012), pp.1-10.
https://search.emarefa.net/detail/BIM-998507
American Medical Association (AMA)
Mitchell, Michael J.& Castellanos, Carlos A.& King, Michael R.. Nanostructured Surfaces to Target and Kill Circulating Tumor Cells While Repelling Leukocytes. Journal of Nanomaterials. 2012. Vol. 2012, no. 2012, pp.1-10.
https://search.emarefa.net/detail/BIM-998507
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998507