Impact of β-Turn Sequence on β-Hairpin Dynamics Studied with Infrared-Detected Temperature Jump
Joint Authors
Popp, Alexander
Wu, Ling
Keiderling, Timothy A.
Hauser, Karin
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Folding dynamics for β-structure loss and disordered structure gain were studied in a model β-hairpin peptide based on Cochran’s tryptophan zipper peptide Trpzip2, but with an altered Thr-Gly (TG) turn sequence, that is, SWTWETGKWTWK, using laser-induced temperature-jump (T-jump) kinetics with IR detection.
As has been shown previously, the TG turn sequence reduces the thermodynamic β-hairpin stability as compared to the Asn-Gly sequence used in Trpzip2 (TZ2-NG).
In this study, we found that the TG-turn slows down the overall relaxation dynamics as compared to TZ2-NG, which were studied at higher temperatures where the time constants show little difference between relaxation of the β-strand and the disordered conformation.
These time constants become equivalent at lower temperatures for TZ2-TG than was seen for TZ2-NG.
The correlation of thermodynamic stability and rates of relaxation suggests that the change from NG to TG turn results in a slowing of folding, lower kf, with less change of the unfolding rate, ku, assuming two state behavior at higher temperatures.
American Psychological Association (APA)
Popp, Alexander& Wu, Ling& Keiderling, Timothy A.& Hauser, Karin. 2012. Impact of β-Turn Sequence on β-Hairpin Dynamics Studied with Infrared-Detected Temperature Jump. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-998724
Modern Language Association (MLA)
Popp, Alexander…[et al.]. Impact of β-Turn Sequence on β-Hairpin Dynamics Studied with Infrared-Detected Temperature Jump. Journal of Spectroscopy No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-998724
American Medical Association (AMA)
Popp, Alexander& Wu, Ling& Keiderling, Timothy A.& Hauser, Karin. Impact of β-Turn Sequence on β-Hairpin Dynamics Studied with Infrared-Detected Temperature Jump. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-998724
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998724