Protein-Ligand Interactions of the D-GalactoseD-Glucose-Binding Protein as a Potential Sensing Probe of Glucose Biosensors
Joint Authors
Stepanenko, Olga V.
Stepanenko, Olesya V.
Fonin, Alexander V.
Verkhusha, Vladislav V.
Kuznetsova, Irina M.
Turoverov, Konstantin K.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
In this paper we have studied peculiarities of protein-ligand interaction under different conditions.
We have shown that guanidine hydrochloride (GdnHCI) unfolding-refolding of GGBP in the presence of glucose (Glc) is reversible, but the equilibrium curves of complex refolding-unfolding have been attained only after 10-day incubation of GGBP/Glc in the presence of GdnHCl.
This effect has not been revealed at heat-induced GGBP/Glc denaturation.
Slow equilibration between the native protein in GGBP/Glc complex and the unfolded state of protein in the GdnHCl presence is connected with increased viscosity of solution at moderate and high GdnHCl concentrations which interferes with diffusion of glucose molecules.
Thus, the limiting step of the unfolding-refolding process of the complex GGBP/Glc is the disruption/tuning of the configuration fit between the protein in the native state and the ligand.
American Psychological Association (APA)
Stepanenko, Olga V.& Stepanenko, Olesya V.& Fonin, Alexander V.& Verkhusha, Vladislav V.& Kuznetsova, Irina M.& Turoverov, Konstantin K.. 2012. Protein-Ligand Interactions of the D-GalactoseD-Glucose-Binding Protein as a Potential Sensing Probe of Glucose Biosensors. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-998736
Modern Language Association (MLA)
Stepanenko, Olga V.…[et al.]. Protein-Ligand Interactions of the D-GalactoseD-Glucose-Binding Protein as a Potential Sensing Probe of Glucose Biosensors. Journal of Spectroscopy No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-998736
American Medical Association (AMA)
Stepanenko, Olga V.& Stepanenko, Olesya V.& Fonin, Alexander V.& Verkhusha, Vladislav V.& Kuznetsova, Irina M.& Turoverov, Konstantin K.. Protein-Ligand Interactions of the D-GalactoseD-Glucose-Binding Protein as a Potential Sensing Probe of Glucose Biosensors. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-998736
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998736