Evaluation of Biochemical Changes in Skin-Derived Mesenchymal Stem Cells during In Vitro Neurodifferentiation by FT-IR Analysis

Abstract EN

Recently FT-IR analysis has been employed to study changes in molecular signatures during embryonic stem cell differentiation.

We were interested to find out whether FT-IR spectroscopy could be applied to analyze changes in human skin mesenchymal stem cell (S-MSC) biochemical profile during in vitro neurodifferentiation.

S-MSCs were propagated in serum-free medium with EGF and FGF-2 during six weeks.

Neural progenitor cell line ReNcell CX (Millipore) was used as a reference cell line.

Samples were collected each week and analyzed for neural marker nestin, tubulin βIII, GFAP, and CD271 expression.

FT-IR analysis was carried out using microplate reader HTS-XT (Bruker, Germany).

Despite the immunophenotype similarity, FT-IR spectroscopy revealed distinct profiles for S-MSC culture and ReNcell CX cells.

FT-IR spectra analyses showed changes of protein and lipid concentration during neurodifferentiation and different carbohydrate composition in ReNcell CX and S-MSCs.

It was possible to discriminate between S-MSC cultures at different time points during neurodifferentiation.

The results of this study demonstrate that FT-IR spectroscopy is more sensitive than conventional immunophenotyping analysis and it has a great potential for the monitoring of the stem cell differentiation status.