SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops
Joint Authors
Kočišová, E.
Procházka, M.
Šimáková, P.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
We report the application of dried Ag hydroxylamine-reduced colloidal drops to surface-enhanced (resonance) Raman scattering (SE(R)RS) study of biomolecules using Raman microspectroscopy.
5,10,15,20-tetrakis(1-methyl-4-pyridyl)porphyrin (TMPyP), amino acid tryptophan, and phospholipid 1,2-distearoyl-sn-glycero-3-phosphocholine (DSPC) served as testing biomolecules.
Ag colloid/biomolecule drop dried on glass support forms a ring in the edge part of the drop in which almost all nanoparticles are clustered.
This specific drying process promotes adsorption of the studied biomolecules in highly enhancing sites (“hot spots”) as well as concentrates them in the ring.
We were able to obtain SE(R)RS spectra from the ring that cannot be acquired directly from Ag colloidal solutions (SERRS spectrum of 1×10−10 M TMPyP by 1 s accumulation time, SERS spectrum of 2×10−7 M DSPC).
Despite the spectral irreproducibility and problems with spurious bands in some cases, SERS microspectroscopy of studied biomolecules using dried Ag colloid/adsorbate systems improves SERS applicability and sensitivity in comparison to measurements directly from Ag colloidal solution.
American Psychological Association (APA)
Šimáková, P.& Procházka, M.& Kočišová, E.. 2012. SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-998750
Modern Language Association (MLA)
Šimáková, P.…[et al.]. SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops. Journal of Spectroscopy No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-998750
American Medical Association (AMA)
Šimáková, P.& Procházka, M.& Kočišová, E.. SERS Microspectroscopy of Biomolecules on Dried Ag Colloidal Drops. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-998750
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998750