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Fluorescence Lifetime Imaging of Propranolol Uptake in Living Glial C6 Cells
Joint Authors
Bisby, Roger
Botchway, Stanley
Crisostomo, Ana
Parker, Anthony
Scherer, Kathrin
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Uptake of the β-blocker drug propranolol by living glial C6 cells has been observed using fluorescence lifetime imaging with two-photon excitation at 630 nm.
Both uptake and release of propranolol occur within minutes and are temperature dependent, being about 5 times faster at 37°C than at 20°C.
The intracellular fluorescence lifetime of propranolol is generally shorter than the value of 9.8 ns determined in dilute neutral aqueous solution, and the difference is ascribed to concentration quenching.
Within the cells, propranolol is accumulated within intracellular acidic vesicles and the cytoplasm but is excluded from the cell nucleus.
On incubation of cells in medium containing 100 μM propranolol, the drug is accumulated to reach intracellular concentrations up to 10 mM in a process that is believed to be driven by protonation within acidic cellular compartments.
American Psychological Association (APA)
Bisby, Roger& Botchway, Stanley& Crisostomo, Ana& Parker, Anthony& Scherer, Kathrin. 2012. Fluorescence Lifetime Imaging of Propranolol Uptake in Living Glial C6 Cells. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-998759
Modern Language Association (MLA)
Bisby, Roger…[et al.]. Fluorescence Lifetime Imaging of Propranolol Uptake in Living Glial C6 Cells. Journal of Spectroscopy No. 2012 (2012), pp.1-8.
https://search.emarefa.net/detail/BIM-998759
American Medical Association (AMA)
Bisby, Roger& Botchway, Stanley& Crisostomo, Ana& Parker, Anthony& Scherer, Kathrin. Fluorescence Lifetime Imaging of Propranolol Uptake in Living Glial C6 Cells. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-8.
https://search.emarefa.net/detail/BIM-998759
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998759