Influence of Fixation Products Used in the Histological Processing in the FTIR Spectra of Lung Cells
Joint Authors
Pereira, Thiago Martini
Dagli, Maria Lucia Zaidan
Mennecier, Grégory
Zezell, Denise Maria
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-4, 4 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
4
Main Subjects
Abstract EN
The aim of the present study is to evaluate the differences on FTIR spectra of the normal lung cell (noncancerous mice lung epithelial cell line e10) due to different fixation protocols for histological processing.
The results shown that formalin and methacarn (normally used in fixation) did cause many changes on the FTIR spectra of mice lung cells e10, mainly in the organic compounds (800–1800 cm−1) in lipids, DNA, and proteins, and the alcohol 70% fixation protocol caused almost no changes on the FTIR spectra compared to unfixed cells spectra (in PBS).
It can be concluded that histological processing with alcohol 70% fixation protocol can be used in the FTIR study of mice lung cell line e10.
American Psychological Association (APA)
Pereira, Thiago Martini& Dagli, Maria Lucia Zaidan& Mennecier, Grégory& Zezell, Denise Maria. 2012. Influence of Fixation Products Used in the Histological Processing in the FTIR Spectra of Lung Cells. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-998769
Modern Language Association (MLA)
Pereira, Thiago Martini…[et al.]. Influence of Fixation Products Used in the Histological Processing in the FTIR Spectra of Lung Cells. Journal of Spectroscopy No. 2012 (2012), pp.1-4.
https://search.emarefa.net/detail/BIM-998769
American Medical Association (AMA)
Pereira, Thiago Martini& Dagli, Maria Lucia Zaidan& Mennecier, Grégory& Zezell, Denise Maria. Influence of Fixation Products Used in the Histological Processing in the FTIR Spectra of Lung Cells. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-4.
https://search.emarefa.net/detail/BIM-998769
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998769
