Spectroscopic Determination of pKa Constants of MADS Box Segments
Joint Authors
Řezáčová, Barbora
Coïc, Yves-Marie
Zentz, Christian
Turpin, Pierre-Yves
Štěpánek, Josef
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
We have introduced a new promising approach for the determination of pKa constants of oligopeptide intrinsic fluorophores and spectral components referring to their differently charged states.
The method is based on the factor analysis of multiwavelength spectroscopic pH titration data.
As an illustration, we present its application on the study of short segments of the MADS box, which is a highly conserved sequence of a so-called family of transcription factors, by techniques of UV absorption and fluorescence spectroscopies.
Investigated oligopeptides contain no tryptophan but one tyrosine serving as an intrinsic fluorophore and absorber.
The results indicate both good sensitivity and spectroscopic selectivity of our method, which thus may be considered as a complementary technique to conventional electrochemical methods.
American Psychological Association (APA)
Řezáčová, Barbora& Coïc, Yves-Marie& Zentz, Christian& Turpin, Pierre-Yves& Štěpánek, Josef. 2012. Spectroscopic Determination of pKa Constants of MADS Box Segments. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-998776
Modern Language Association (MLA)
Řezáčová, Barbora…[et al.]. Spectroscopic Determination of pKa Constants of MADS Box Segments. Journal of Spectroscopy No. 2012 (2012), pp.1-7.
https://search.emarefa.net/detail/BIM-998776
American Medical Association (AMA)
Řezáčová, Barbora& Coïc, Yves-Marie& Zentz, Christian& Turpin, Pierre-Yves& Štěpánek, Josef. Spectroscopic Determination of pKa Constants of MADS Box Segments. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-7.
https://search.emarefa.net/detail/BIM-998776
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998776