Raman Microspectroscopy of the Yeast Vacuoles
Joint Authors
Bednárová, Lucie
Palacký, Jan
Bauerová, Václava
Hrušková-Heidingsfeldová, Olga
Pichová, Iva
Mojzeš, Peter
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
In the present work, real ability of a confocal Raman microspectroscopy to monitor chemical composition of the vacuoles within living yeast cells was investigated and critically assessed.
Simple, economical, and practical protocols of the yeast immobilization suitable for less laborious, high-throughput, and spatially resolved Raman measurements were tested for their possible impacts on physiological states and viability of the cells.
We have demonstrated that, acquiring Raman spectra from statistically sound sets of immobilized cells and employing advanced multivariate methods for spectral analysis, the chemical composition of the yeast vacuoles can be reliably studied.
The most easily and accurately quantifiable seems to be the concentration of polyphosphates which can be unambiguously identified due to unmistakable Raman features.
Our approach can be useful for routine, label-free, and noninvasive monitoring of the chemical composition of the vacuoles of living yeasts exposed to various stress factors, the information important in biomedical research of pathogens.
American Psychological Association (APA)
Bednárová, Lucie& Palacký, Jan& Bauerová, Václava& Hrušková-Heidingsfeldová, Olga& Pichová, Iva& Mojzeš, Peter. 2012. Raman Microspectroscopy of the Yeast Vacuoles. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-998792
Modern Language Association (MLA)
Bednárová, Lucie…[et al.]. Raman Microspectroscopy of the Yeast Vacuoles. Journal of Spectroscopy No. 2012 (2012), pp.1-5.
https://search.emarefa.net/detail/BIM-998792
American Medical Association (AMA)
Bednárová, Lucie& Palacký, Jan& Bauerová, Václava& Hrušková-Heidingsfeldová, Olga& Pichová, Iva& Mojzeš, Peter. Raman Microspectroscopy of the Yeast Vacuoles. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-5.
https://search.emarefa.net/detail/BIM-998792
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998792