A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins

Joint Authors

Malferrari, Marco
Venturoli, Giovanni
Francia, Francesco
Mezzetti, Alberto

Source

Journal of Spectroscopy

Issue

Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2012-07-11

Country of Publication

Egypt

No. of Pages

6

Main Subjects

Physics

Abstract EN

In this paper, we describe a new method to obtain D2O/H2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides.

The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample.

The proposed method can be applied to other proteins.

American Psychological Association (APA)

Malferrari, Marco& Venturoli, Giovanni& Francia, Francesco& Mezzetti, Alberto. 2012. A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-998796

Modern Language Association (MLA)

Malferrari, Marco…[et al.]. A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins. Journal of Spectroscopy No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-998796

American Medical Association (AMA)

Malferrari, Marco& Venturoli, Giovanni& Francia, Francesco& Mezzetti, Alberto. A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-998796

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-998796