A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins
Joint Authors
Malferrari, Marco
Venturoli, Giovanni
Francia, Francesco
Mezzetti, Alberto
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-11
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
In this paper, we describe a new method to obtain D2O/H2O exchange in photosynthetic reaction centres from Rhodobacter sphaeroides.
The method is characterized by: (i) a very high efficiency of the isotopic replacement; (ii) an extremely low amount of D2O needed; (iii) the short time required for dehydration and D2O rehydration; (iv) the possibility of controlling concomitantly the hydration state of the sample.
The proposed method can be applied to other proteins.
American Psychological Association (APA)
Malferrari, Marco& Venturoli, Giovanni& Francia, Francesco& Mezzetti, Alberto. 2012. A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins. Journal of Spectroscopy،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-998796
Modern Language Association (MLA)
Malferrari, Marco…[et al.]. A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins. Journal of Spectroscopy No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-998796
American Medical Association (AMA)
Malferrari, Marco& Venturoli, Giovanni& Francia, Francesco& Mezzetti, Alberto. A New Method for D2OH2O Exchange in Infrared Spectroscopy of Proteins. Journal of Spectroscopy. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-998796
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998796