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LPS Counter Regulates RNA Expression of Extracellular Proteases and Their Inhibitors in Murine Macrophages
Joint Authors
Egerod, Kristoffer L.
Hald, Andreas
Rønø, Birgitte
Lund, Leif R.
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-03-14
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Besides their evident importance in host defense, macrophages have been shown to play a detrimental role in different pathological conditions, including chronic inflammation, atherosclerosis, and cancer.
Regardless of the exact situation, macrophage activation and migration are intimately connected to extracellular matrix degradation.
This process is accomplished by multiple proteolytic enzymes, including serine proteases and members of the matrix metalloproteinase family.
In this study, we have utilized qPCR arrays to simultaneously analyze the temporal expression pattern of a range of genes involved in extracellular matrix metabolism in the mouse derived-macrophage cell line RAW 264.7 following stimulation with LPS.
Our results revealed that LPS induces the expression of matrix metalloproteinases while at the same time decreased the expression of matrix metalloproteinase inhibitors.
The opposite scenario was found for the genes encoding serine proteases, which were downregulated while their inhibitors were upregulated.
In addition, intergenic comparison of the expression levels of related proteases revealed large differences in their basal expression level.
These data highlight the complexity of the gene expression regulation implicated in macrophage-dependent matrix degradation and furthermore emphasize the value of qPCR array techniques for the investigation of the complex regulation of the matrix degradome.
American Psychological Association (APA)
Hald, Andreas& Rønø, Birgitte& Lund, Leif R.& Egerod, Kristoffer L.. 2012. LPS Counter Regulates RNA Expression of Extracellular Proteases and Their Inhibitors in Murine Macrophages. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-998838
Modern Language Association (MLA)
Hald, Andreas…[et al.]. LPS Counter Regulates RNA Expression of Extracellular Proteases and Their Inhibitors in Murine Macrophages. Mediators of Inflammation No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-998838
American Medical Association (AMA)
Hald, Andreas& Rønø, Birgitte& Lund, Leif R.& Egerod, Kristoffer L.. LPS Counter Regulates RNA Expression of Extracellular Proteases and Their Inhibitors in Murine Macrophages. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-998838
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998838