Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells
Joint Authors
Shimizu, Takeshi
Shimizu, Shino
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-04-23
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
To examine the in vivo effects of the 15-member macrolide, azithromycin (AZM), on mucus hypersecretion, we induced hypertrophic and metaplastic changes of goblet cells in rat nasal epithelium by intranasal instillation of ovalbumin (OVA) in OVA-sensitized rats, or by intranasal lipopolysaccharides (LPS) instillation.
Oral administration of AZM (5–10 mg/kg) or clarithromycin (CAM, 5–10 mg/kg) significantly inhibited OVA- and LPS-induced mucus production, whereas josamycin (JM) or ampicillin (ABPC) showed no effect.
In vitro effects of AZM on airway epithelial cells were examined using NCI-H292 cells and human nasal epithelial cells cultured in air-liquid interface.
Mucus secretion was evaluated by enzyme-linked immunosorbent assay using an anti-MUC5AC monoclonal antibody.
AZM or CAM significantly inhibited tumor necrosis factor-α (TNF-α) (20 ng/mL)-induced MUC5AC secretion from NCI-H292 cells at 10−6–10−7 M, whereas JM or ABPC showed no effect.
AZM significantly inhibited TNF-α (20 ng/mL)-induced MUC5AC secretion from human nasal epithelial cells at 10−4 M.
MUC5AC mRNA expression was also significantly inhibited.
These results indicate that the 15-member macrolide, AZM, exerts direct inhibitory effects on mucus secretion from airway epithelial cells and that it may be useful for the treatment of mucus hypersecretion caused by allergic inflammation and LPS stimulation.
American Psychological Association (APA)
Shimizu, Takeshi& Shimizu, Shino. 2012. Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-998865
Modern Language Association (MLA)
Shimizu, Takeshi& Shimizu, Shino. Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells. Mediators of Inflammation No. 2012 (2012), pp.1-6.
https://search.emarefa.net/detail/BIM-998865
American Medical Association (AMA)
Shimizu, Takeshi& Shimizu, Shino. Azithromycin Inhibits Mucus Hypersecretion from Airway Epithelial Cells. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-6.
https://search.emarefa.net/detail/BIM-998865
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998865