MCP-1-Induced Histamine Release from Mast Cells Is Associated with Development of Interstitial CystitisBladder Pain Syndrome in Rat Models
Joint Authors
Lv, Jianwei
Huang, Yiran
Zhu, Shiguo
Yang, Ganggang
Zhang, Yujian
Leng, Jing
Bo, Juanjie
Liu, Dongming
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-09-19
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Interstitial cystitis/bladder pain syndrome (IC/BPS) is characterized by overexpression of monocyte chemoattractant protein-1 (MCP-1) in bladder tissues and induction of mast cell (MC) degranulation.
This study was undertaken to explore the mechanism of action of MCP-1 in the development of IC/BPS.
Methods.
A rat model of IC/BPS was developed by perfusing bladders of nine SPF- grade female Sprague-Dawley rats with protamine sulfate and lipopolysaccharide (PS+LPS).
MCP-1 and histamine levels in bladder tissue and urine were detected by immunohistochemistry and ELISA.
MC degranulation was measured by immunofluorescence techniques and chemokine (C-C motif) receptor 2 (CCR2) was assayed by flow cytometry.
Results.
Increased MCP-1 expression in bladder tissue and elevated MCP-1 and histamine levels were observed in the urine of LS+LPS-treated rats.
This was accompanied by the expression of CCR2 on MC surfaces, suggesting MCP-1 may induce MC degranulation through CCR2.
Exposure to LPS stimulated MCP-1 expression in bladder epithelial cells, and exposure to MCP-1 induced histamine release from MCs.
Conclusions.
MCP-1 upregulation in IC/BPS is one of possible contributing factors inducing histamine release from MCs.
CCR2 is involved in the process of mast cell degranulation in bladder tissues.
These changes may contribute to the development of symptoms of IC/BPS.
American Psychological Association (APA)
Lv, Jianwei& Huang, Yiran& Zhu, Shiguo& Yang, Ganggang& Zhang, Yujian& Leng, Jing…[et al.]. 2012. MCP-1-Induced Histamine Release from Mast Cells Is Associated with Development of Interstitial CystitisBladder Pain Syndrome in Rat Models. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-998882
Modern Language Association (MLA)
Lv, Jianwei…[et al.]. MCP-1-Induced Histamine Release from Mast Cells Is Associated with Development of Interstitial CystitisBladder Pain Syndrome in Rat Models. Mediators of Inflammation No. 2012 (2012), pp.1-9.
https://search.emarefa.net/detail/BIM-998882
American Medical Association (AMA)
Lv, Jianwei& Huang, Yiran& Zhu, Shiguo& Yang, Ganggang& Zhang, Yujian& Leng, Jing…[et al.]. MCP-1-Induced Histamine Release from Mast Cells Is Associated with Development of Interstitial CystitisBladder Pain Syndrome in Rat Models. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-9.
https://search.emarefa.net/detail/BIM-998882
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998882