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Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation
Joint Authors
Yui, Satoru
Morimoto-Kamata, Riyo
Mizoguchi, Sei-ichiro
Ichisugi, Takeo
Source
Issue
Vol. 2012, Issue 2012 (31 Dec. 2012), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2012-07-08
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Neutrophils often invade various tumor tissues and affect tumor progression and metastasis.
Cathepsin G (CG) is a serine protease secreted from activated neutrophils.
Previously, we have shown that CG induces the formation of E-cadherin-mediated multicellular spheroids of human breast cancer MCF-7 cells; however, the molecular mechanisms involved in this process are unknown.
In this study, we investigated whether CG required its enzymatic activity to induce MCF-7 cell aggregation.
The cell aggregation-inducing activity of CG was inhibited by pretreatment of CG with the serine protease inhibitors chymostatin and phenylmethylsulfonyl fluoride.
In addition, an enzymatically inactive S195G (chymotrypsinogen numbering) CG did not induce cell aggregation.
Furthermore, CG specifically bound to the cell surface of MCF-7 cells via a catalytic site-independent mechanism because the binding was not affected by pretreatment of CG with serine protease inhibitors, and cell surface binding was also detected with S195G CG.
Therefore, we propose that the CG-induced aggregation of MCF-7 cells occurs via a 2-step process, in which CG binds to the cell surface, independently of its catalytic site, and then induces cell aggregation, which is dependent on its enzymatic activity.
American Psychological Association (APA)
Morimoto-Kamata, Riyo& Mizoguchi, Sei-ichiro& Ichisugi, Takeo& Yui, Satoru. 2012. Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation. Mediators of Inflammation،Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-998956
Modern Language Association (MLA)
Morimoto-Kamata, Riyo…[et al.]. Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation. Mediators of Inflammation No. 2012 (2012), pp.1-13.
https://search.emarefa.net/detail/BIM-998956
American Medical Association (AMA)
Morimoto-Kamata, Riyo& Mizoguchi, Sei-ichiro& Ichisugi, Takeo& Yui, Satoru. Cathepsin G Induces Cell Aggregation of Human Breast Cancer MCF-7 Cells via a 2-Step Mechanism: Catalytic Site-Independent Binding to the Cell Surface and Enzymatic Activity-Dependent Induction of the Cell Aggregation. Mediators of Inflammation. 2012. Vol. 2012, no. 2012, pp.1-13.
https://search.emarefa.net/detail/BIM-998956
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-998956